Elkalawy, S., Abo Gazia, M., Abd Elaziz Elekhtiar, S., Ibrahim Ismail, D. (2020). Collagen Hydrolysate Against Fluvoxamine Maleate-Induced Osteoporosis in Albino Rats: A Histological and Immunohistochemical Study. Egyptian Journal of Histology, 43(4), 988-1007. doi: 10.21608/ejh.2020.22975.1239
Seham Abd Elhamid Elkalawy; Maha Mohamed Abo Gazia; Sally Ahmed Abd Elaziz Elekhtiar; Dalia Ibrahim Ismail. "Collagen Hydrolysate Against Fluvoxamine Maleate-Induced Osteoporosis in Albino Rats: A Histological and Immunohistochemical Study". Egyptian Journal of Histology, 43, 4, 2020, 988-1007. doi: 10.21608/ejh.2020.22975.1239
Elkalawy, S., Abo Gazia, M., Abd Elaziz Elekhtiar, S., Ibrahim Ismail, D. (2020). 'Collagen Hydrolysate Against Fluvoxamine Maleate-Induced Osteoporosis in Albino Rats: A Histological and Immunohistochemical Study', Egyptian Journal of Histology, 43(4), pp. 988-1007. doi: 10.21608/ejh.2020.22975.1239
Elkalawy, S., Abo Gazia, M., Abd Elaziz Elekhtiar, S., Ibrahim Ismail, D. Collagen Hydrolysate Against Fluvoxamine Maleate-Induced Osteoporosis in Albino Rats: A Histological and Immunohistochemical Study. Egyptian Journal of Histology, 2020; 43(4): 988-1007. doi: 10.21608/ejh.2020.22975.1239
Collagen Hydrolysate Against Fluvoxamine Maleate-Induced Osteoporosis in Albino Rats: A Histological and Immunohistochemical Study
1Histology Department, Faculty of Medicine, Cairo University, Cairo, Egypt
2Histology department Faculty of Medicine,Khafrelskeikh University, Khafrelskeikh, Egypt
3Histology Department, Faculty of Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt
Abstract
Background: Osteoporosis (OP) is a prevalent degenerative bone disease among patients receiving selective serotonin reuptake inhibitors as fluvoxamine maleate (FM). Collagen hydrolysate (CH) is a nutritional component that has antiresorptive effect. Aim of Work: Evaluate the possible protective effect of CH against FM-induced OP in adult male albino rats. Material and Methods: Thirty six rats were divided into 4 groups; group I (control), group II (OP group): injected with FM daily for 4 weeks, group III (CH group): received FM concomitant with oral CH for 4 weeks, group IV (recovery group]: received only FM for 4 weeks & were left without taking any drugs for another 4 weeks. Total serum alkaline phosphatase (ALP) and calcium (ca+2) were measured. Bone specimens from the right femurs and first lumbar vertebrae were processed for H&E stain, Mallory's trichrome stain and immunohistochemical staining for osteopontin (OPN) and proliferating cell nuclear antigen (PCNA). This was followed by morphometric & statistical analysis. Results: Both groups II & IV showed significant elevation in ALP & reduction in Ca+2 compared to control. Bone sections revealed evident histological changes; osteocytes with pyknotic nuclei inside widened lacuna, widened haversian canals. Bone matrix showed fain areas, cavitations & multiple resorption cavities with osteoclasts. There was significant reduction in the mean thickness of compact bone, the mean area of trabecular bone, area % of OPN & mean number of PCNA +ve cells compared to control. Group III exhibited significant reduction in ALP & elevation in Ca+2. The bone showed preserved histological architecture almost as the control. There was significant increase in the mean thickness of compact bone, the mean area of trabecular bone, area % of OPN & mean number of PCNA +ve cells compared to groups II & IV. Conclusion: CH has a potential osteoprotective effect against FM-induced osteoporosis
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