Collagen Hydrolysate Against Fluvoxamine Maleate-Induced Osteoporosis in Albino Rats: A Histological and Immunohistochemical Study

Document Type : Original Article

Authors

1 Histology Department, Faculty of Medicine, Cairo University, Cairo, Egypt

2 Histology department Faculty of Medicine,Khafrelskeikh University, Khafrelskeikh, Egypt

3 Histology Department, Faculty of Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt

Abstract

Background: Osteoporosis (OP) is a prevalent degenerative bone disease among patients receiving selective serotonin reuptake inhibitors as fluvoxamine maleate (FM). Collagen hydrolysate (CH) is a nutritional component that has antiresorptive effect.
Aim of Work: Evaluate the possible protective effect of CH against FM-induced OP in adult male albino rats.
Material and Methods: Thirty six rats were divided into 4 groups; group I (control), group II (OP group): injected with FM daily for 4 weeks, group III (CH group): received FM concomitant with oral CH for 4 weeks, group IV (recovery group]: received only FM for 4 weeks & were left without taking any drugs for another 4 weeks. Total serum alkaline phosphatase (ALP) and calcium (ca+2) were measured. Bone specimens from the right femurs and first lumbar vertebrae were processed for H&E stain, Mallory's trichrome stain and immunohistochemical staining for osteopontin (OPN) and proliferating cell nuclear antigen (PCNA). This was followed by morphometric & statistical analysis.
Results: Both groups II & IV showed significant elevation in ALP & reduction in Ca+2 compared to control. Bone sections revealed evident histological changes; osteocytes with pyknotic nuclei inside widened lacuna, widened haversian canals. Bone matrix showed fain areas, cavitations & multiple resorption cavities with osteoclasts. There was significant reduction in the mean thickness of compact bone, the mean area of trabecular bone, area % of OPN & mean number of PCNA +ve cells compared to control. Group III exhibited significant reduction in ALP & elevation in Ca+2. The bone showed preserved histological architecture almost as the control. There was significant increase in the mean thickness of compact bone, the mean area of trabecular bone, area % of OPN & mean number of PCNA +ve cells compared to groups II & IV.
Conclusion: CH has a potential osteoprotective effect against FM-induced osteoporosis

Keywords

Egyptian Journal of Histology
Volume 43, Issue 4
December 2020
Pages 988-1007

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