Ahmed, N., Ghannam, H., Tayel, S. (2020). Biochemical and Histopathological Responses of Oreochromis Niloticus and Cyprinus Carpio to Sub-lethal Exposure of Ictacrune Pesticide. Egyptian Journal of Histology, 43(3), 918-930. doi: 10.21608/ejh.2020.21602.1224
Nasr Mohamed Ahmed; Hala Elshahat Ghannam; Safaa Ismail Tayel. "Biochemical and Histopathological Responses of Oreochromis Niloticus and Cyprinus Carpio to Sub-lethal Exposure of Ictacrune Pesticide". Egyptian Journal of Histology, 43, 3, 2020, 918-930. doi: 10.21608/ejh.2020.21602.1224
Ahmed, N., Ghannam, H., Tayel, S. (2020). 'Biochemical and Histopathological Responses of Oreochromis Niloticus and Cyprinus Carpio to Sub-lethal Exposure of Ictacrune Pesticide', Egyptian Journal of Histology, 43(3), pp. 918-930. doi: 10.21608/ejh.2020.21602.1224
Ahmed, N., Ghannam, H., Tayel, S. Biochemical and Histopathological Responses of Oreochromis Niloticus and Cyprinus Carpio to Sub-lethal Exposure of Ictacrune Pesticide. Egyptian Journal of Histology, 2020; 43(3): 918-930. doi: 10.21608/ejh.2020.21602.1224
Biochemical and Histopathological Responses of Oreochromis Niloticus and Cyprinus Carpio to Sub-lethal Exposure of Ictacrune Pesticide
National Institute of Oceanography and Fisheries, Cairo, Egypt
Abstract
Aim of the work: Determining the negative impact of sub-lethal concentrations on biochemical and histological of Oreochromis niloticus and Cyprinus carpio. Introduction: Ictacrune pesticide (Profenofos), is an organophosphate pesticide over the last two decades were used in agricultural for controlling pests. The toxicity of profenofos is the inhibition of the acetylcholine esterase activity resulting in neuro toxicity to aquatic vertebrates and humans. Results and Discussion: Oreochromis niloticus and Cyprinus carpio were subjected to different concentrations (0.036 – 0.252 mg/l) and (0.0072-0.108 mg/l), respectively, of Ictacrune pesticide for 96 hours andthe lethal concentration (LC50)values of Ictacrune pesticide for the two studied fishes were 0.144 and 0.02662 mg/l Ictacrune, respectively, for 96 hours of exposure. The values of lipid, glucose, T. protein and albumin were significantly (P<0.05) decreased for Cyprinus carpio from (650.6, 85.5 mg/dl, 3.2 and1.5 g/dl, respectively), for control group to 255.3, 42.1 mg/dl, 2.15 and 0.95 g/dl and 158.15, 31.6 mg/dl, 2.15 and 0.85 g/dl after 45 days exposure for 0.25&0.50 LC50, while, O. niloticus (870.0, 88.50 mg/dl, 3.60 a3nd 1.90 g/dl, respectively for control group to 285.0, 55.9 mg/dl, 2.45 and 0.90 g/dl and 195.0, 44.55 mg/dl, 2.15 and 0.80 g/dl after 45 days exposure for 0.25&0.50 LC50 of Ictacrune pesticide. The values ALT, AST, urea and uric acid were significantly (P<0.05) increased for O. niloticus from 15.30, 33.24 IU/ml, 25.0 and 4.0 mg/dl, respectively, of control group to 51.70, 94.40 IU/ml, 162.90 and 21.90 mg/dl and 67.50, 112.35 IU/ml, 194.30 and 24.60 mg/dl, respectively, after 45 days exposure for 0.25&0.50 LC50 of Ictacrune pesticide. While as these values were significantly (P<0.05) increased for Cyprinus carpio, from (17.30, 39.24 IU/ml, 45.50 and 6.50 mg/dl), respectively, in control group to (87.50, 107.40 IU/ml, 123.10 and 25.50 mg/dl) and (92.75, 132.35 IU/ml, 142.80 and 30.90 mg/dl), respectively, after 45 days exposure for 0.25&0.5 LC50 of Ictacrune pesticide. The histological examination of the liver, muscles and gills obtained from the two studied fishes exposed to Ictacrune pesticide for 45 days in present study revealed many alterations including, degeneration, necrosis, piknosis, congestion, edema, fibrosis, hyperplasia, curling, separation and hemorrhage. The degree of these alterations depended on dose and the period of Ictacrune pesticide exposure. Conclusion: there is a direct relationship between pesticide exposure and histopathological alterations that observed in studied organs. The degree of these alterations depended on dose and the period of Ictacrune pesticide exposure.
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