Abdel Moneim, R., Mostafa, A., Abbass, M. (2020). In Treating Glucocorticoids Induced Osteoporosis in Temporomandibular Joint of Albino Rats; Which are More Effective Microvesicles or Mesenchymal Stem Cells?. Egyptian Journal of Histology, 43(3), 849-862. doi: 10.21608/ejh.2020.20936.1213
Rehab Ali Abdel Moneim; Abeer Mostafa; Marwa Magdy Saad Abbass. "In Treating Glucocorticoids Induced Osteoporosis in Temporomandibular Joint of Albino Rats; Which are More Effective Microvesicles or Mesenchymal Stem Cells?". Egyptian Journal of Histology, 43, 3, 2020, 849-862. doi: 10.21608/ejh.2020.20936.1213
Abdel Moneim, R., Mostafa, A., Abbass, M. (2020). 'In Treating Glucocorticoids Induced Osteoporosis in Temporomandibular Joint of Albino Rats; Which are More Effective Microvesicles or Mesenchymal Stem Cells?', Egyptian Journal of Histology, 43(3), pp. 849-862. doi: 10.21608/ejh.2020.20936.1213
Abdel Moneim, R., Mostafa, A., Abbass, M. In Treating Glucocorticoids Induced Osteoporosis in Temporomandibular Joint of Albino Rats; Which are More Effective Microvesicles or Mesenchymal Stem Cells?. Egyptian Journal of Histology, 2020; 43(3): 849-862. doi: 10.21608/ejh.2020.20936.1213
In Treating Glucocorticoids Induced Osteoporosis in Temporomandibular Joint of Albino Rats; Which are More Effective Microvesicles or Mesenchymal Stem Cells?
1Oral biology department, faculty of dentistry, Cairo university and Future university in Egypt
2Medical Biochemistry and Molecular Biology, Faculty of medicine, Cairo University
36 Bahget st. Elnozha, Heliopolis
Abstract
Background: Osteoporosis is a systemic skeletal disorder that increases bone fragility and risk of fractures. Current medications for treating osteoporosis are associated with osteonecrosis due to their anti-angiogenic effect that leads to ischaemic and necrotic changes. Aim: The present research compared two new approaches in treating glucocorticoids induced osteoporosis in temporomandibular joints (TMJs) of albino rats; Microvesicles (MVs) and Mesenchymal stem cells (MSCs). Materials and Methods: 39 adult female albino rats were divided into 3 groups. All rats were intraperitoneally injected once daily throughout the experimental period (60 days); 13 rats with 9% saline and 26 rats with dexamethasone (200μg/100 g body weight). After 30 days; the first group (control group) received a local injection of 1 ml PBS in TMJs of both sides. Half the dexamethasone injected rats were locally injected in the right side TMJs with 1x107/ml/week MVs (Microvesicles group). The remaining rats (Stem cells group) received 1 million MSCs/ ml/week. In the later 2 groups, the left side TMJs received 1 ml PBS injection (untreated sides). The osteogenic potential was examined histologically, by western blotting, ELISA and RT-PCR. Results: Histologically; both sides from MSCs group exhibited abnormal configuration of the joint’s anatomy while a marked improvement of bone architecture in the microvesicles treated TMJs was detected. Treated sides in both groups (MVs, MSCs) showed a significant increase in osteogenic markers (ALP, BMP and RUNX-2) and a decrease in inflammatory markers (IL-1β, TNF-α, IL-6) as well as in RANKL expression as compared to untreated ones. Conclusion: Microvesicles treated TMJs showed an enhanced pattern of bone regeneration confirmed by the higher mean bone area % as compared to MSCs group. On the contrary, MSCs treated TMJs showed a significantly elevated level of osteogenic markers; yet the osteogenic reactivity was very aggressive that caused deformity to the TMJ architecture.
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