Effects of Nicotine Administration on the Structure of Auditory Cortex of Adolescent Male Guinea Pigs, a Histological and Ultrastructural Study

Othman, Manal A; Oseily, Amira M; Ramadan, Enas M

doi:10.21608/ejh.2019.16780.1165

Effects of Nicotine Administration on the Structure of Auditory Cortex of Adolescent Male Guinea Pigs, a Histological and Ultrastructural Study

Document Type : Original Article

Authors

¹ Dept. of Anatomy, College of Medicine-Arabian Gulf University Bahrain &amp; Dept. of Histology, Faculty of Medicine, Assiut University, Egypt

² Dept. of Ear, Nose &amp; Throat, Faculty of Medicine, Assiut University, Egypt

10.21608/ejh.2019.16780.1165

Abstract

Background: Nicotine, the main ingredient in tobacco smoke, has always been linked to degenerative changes to the nervous system and several areas in the brain were reported to be injured due to nicotine. The effect of nicotine on the auditory system is only being recognized recently with few studies assessed the morphology. The effect of nicotine on the primary auditory cortex of young adolescent animals was addressed in this study.
Materials and Methods: Twenty young male guinea pigs of two months old were divided into two groups of 10 animals each. Group I, the control group, received daily subcutaneous injections of normal saline for one month. Group II, the nicotine-treated group, received 3 mg/Kg body weight of nicotine subcutaneously daily. After animal sacrifice, brains were removed and processed for light and electron microscopic evaluations. Morphometry was also done to light microscopic histological sections.
Results: In the nicotine-treated group, there were degenerative changes affecting the neurons, glia as well as blood capillaries. There was a darkening of neurons and disruption of their dendrites and organelles. The glial cells revealed reactivity, swelling, and cytoplasmic disruption. Blood capillaries showed collapse and thickening of their basement membrane. Morphometry revealed that the thickness of the auditory cortex has decreased as well as the dark neuronal number has increased in the treated group versus the control.
Conclusion: Nicotine administration to adolescent male guinea pigs resulted in degenerative changes affecting the auditory cortex of the brain, which emphasizes the hazardous effects of cigarette smoking, especially at a young age.

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