Mazen, N., Saleh, E., Mahmoud, A., Shaalan, A. (2017). Histological and immunohistochemical study on the potential toxicity of sliver nanoparticles on the structure of the spleen in adult male albino rats. Egyptian Journal of Histology, 40(3), 374-387. doi: 10.21608/EJH.2017.4662
Nehad F. Mazen; Ebtehal Z. Saleh; Abeer A. Mahmoud; Aly A. Shaalan. "Histological and immunohistochemical study on the potential toxicity of sliver nanoparticles on the structure of the spleen in adult male albino rats". Egyptian Journal of Histology, 40, 3, 2017, 374-387. doi: 10.21608/EJH.2017.4662
Mazen, N., Saleh, E., Mahmoud, A., Shaalan, A. (2017). 'Histological and immunohistochemical study on the potential toxicity of sliver nanoparticles on the structure of the spleen in adult male albino rats', Egyptian Journal of Histology, 40(3), pp. 374-387. doi: 10.21608/EJH.2017.4662
Mazen, N., Saleh, E., Mahmoud, A., Shaalan, A. Histological and immunohistochemical study on the potential toxicity of sliver nanoparticles on the structure of the spleen in adult male albino rats. Egyptian Journal of Histology, 2017; 40(3): 374-387. doi: 10.21608/EJH.2017.4662
Histological and immunohistochemical study on the potential toxicity of sliver nanoparticles on the structure of the spleen in adult male albino rats
1Department of Histology and Cell Biology, Faculty of Medicine, Zagazig University1
2Department of Histology and Cell Biology, Faculty of Medicine, Zagazig University
3Department of Histology and Cell Biology, Faculty of Medicine, Suez Canal University
Abstract
Introduction: Silver nanoparticles (AgNPs) remain one of the controversial research areas regarding their distribution, dissolution and their toxicity to biological systems. Aim of the work: To evaluate the potential toxicity of different doses of AgNPs on the spleen of adult Albino rats. Materials and Methods: Forty adult male albino rats were divided into two main groups; control group: included ten rats that received intraperitoneal saline solution, daily, and experimental group: included thirty rats that received intraperitoneal AgNPs dissolved in saline solution, and were equally subdivided into three subgroups. Subgroup IIa received AgNPs (100 mg/kg/d), subgroup IIb received AgNPs (500 mg/kg/d) and subgroup IIc received AgNPs (1000 mg/kg/d). After 28 days, rats were euthanized and the spleen specimens were prepared for light and electron microscope studies. Results: Spleen of AgNPs-treated rats revealed dose-dependent structural changes in the form of reduced sized white pulp follicles with depletion of lymphocytes, degeneration and apoptosis. Nanoparticles were seen in both white and red pulps. There was a highly statistical significant increase in the optical density of caspase-3 in subgroups IIa, IIb and IIc when compared to that of the control group. Conclusions: AgNPs induced structural alterations in the spleen with evidence of inflammatory response and oxidative stress. These effects were dose-dependent.
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