The effect of daidzein on alendronate induced changes on the lingual mucosa of rats: Biochemical, histological, and scanning electron microscopic study

Document Type : Original Article

Authors

1 Histology Department, Faculty of Medicine, Tanta University, Egypt.

2 Medical Histology and Cell Biology, Faculty of medicine, Zagazig University, Zagazig, Egypt

Abstract

Introduction: Alendronate (AL) is one of the bisphosphonate group that contains nitrogen. It has been used in the treatment of bone disorders but, with other organ side effects. Daidzein (DZ) is one of the isoflavones possessing an antioxidant, anti-inflammatory, and anti-apoptotic effects.
Aim of the Work: Was to investigate daidzein effect on alendronate induced changes on the lingual mucosa of rats through biochemical, histological and scanning electron microscopic evaluation.
Materials and Methods: 25 adult male albino rats; 3-5 months; 180- 220 gm were divided into; Control, AL group: AL (0.05 mg/kg), AL+DZLD: AL + DZ low dose (50 mg/kg), AL+ DZHD: AL + DZ high dose (100 mg/kg). Doses were given orally for twelve weeks, then tongue specimens were processed for the biochemical, light and scanning electron microscopic studies.
Results: AL group displayed a significant increase of tissue malondialdehyde, while a significant decrease of tissue superoxide dismutase. Besides, disorganized, ill-defined and distorted lingual papillae, vacuolated epithelial cell’ cytoplasm with pyknotic nuclei, lost epithelial ridges, congested apparently dilated blood vessels, and significantly decreased mean height and width of the filiform and fungiform papillae by H&E. While, a significant decrease of the optical density of collagen fibers, and E-cadherin. Also, desquamated irregularly arranged atrophied filiform papillae with wide separation, and rough upper surface fungiform with ill-defined taste pores by scanning electron microscope. AL treated group by DZ showed dose dependent improvement of the previously mentioned findings.
Conclusion: Daidzein dose dependently ameliorated alendronate induced changes on the lingual mucosa of rats thru its antioxidant and anti-inflammatory actions.

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