The Potential Hepatoprotective role of Resveratrol in ameliorating the Zinc Oxide Nanoparticles Induced Hepatic Tissue Injury in Rats: A Histological and Immunochemical Study

Document Type : Original Article

Authors

1 Anatomy and Embryology department Faculty of Medicine Ain-Shams University

2 Anatomy and Embryology department Faculty of Medicine Ain_Shams University

3 Department of Anatomy and Embryology, Faculty of Medicine, Ain Shams University, Cairo, Egypt

4 Anatomy and Embryology Faculty of Medicine Ain-Shams University

Abstract

Introduction: Zinc oxide nanoparticles are widely used in industries and medications. This elevated the chances of exposure which may have harmful effects on different body organs as the liver. So it was of a great value to search for natural protective element against this effect.
Aim of the Work: Determine the effect of ZnO NPs on the structure and function of the liver of albino rats and to evaluate the possible protective role of Resveratrol.
Material and Methods: 40 adult male albino rats, were divided into four groups 10 rats each; Group I (Control group), Group II (ZnO NPs group), Group III (Resveratrol group) and Group IV (ZnO NPs + Resveratrol group) with administration of drugs by oral gavage for four weeks.
At the end of the experiment, blood samples were collected for measuring liver enzymes. Livers were harvested and processed for light microscopic examination.
Results: Liver sections from (Group II) showed disorganized hepatic architecture, hepatocytes with darkly stained nuclei, and deep acidophilic cytoplasm were observed. Central veins and sinusoids were congested. The portal tracts were expanded with congested portal vein and proliferation of the bile ducts. An increase in the collagen deposition around the portal tracts and depletion of glycogen content within the cytoplasm. Hepatocyte apoptosis was evident.
Sections of (Group IV) showed the restoration of normal hepatic lobules where most hepatocytes appeared normal, while few appeared with pyknotic nuclei. Most of the sinusoids and portal tracts returned to their normal sizes. Sections showed minimal collagen deposition. Also Resveratrol restored the glycogen content depleted in (Group II). Resveratrol reduced the necrosis of hepatocytes.
Deterioration of liver enzymes was observed in (Group II), with marked improvement in (Group IV).
Conclusion: Resveratrol can protect against the degenerative effect of ZnO NPs on the histological structure and function of the liver.

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