Rashad, H., Ibrahim, N., Ahmed, S., Abd el all, M., Sadek, N. (2023). Histological Study to Compare the Effect of Atomoxetine Versus Formetrol on Dexamethasone-Induced Skeletal Muscle Atrophy in Male mice. Egyptian Journal of Histology, (), -. doi: 10.21608/ejh.2023.240872.1958
Heba Essam Rashad; Noha Abd ellatif Ibrahim; Sarwat Lotfi Ahmed; Marwa Omar Abd el all; Nehad Ahmed Sadek. "Histological Study to Compare the Effect of Atomoxetine Versus Formetrol on Dexamethasone-Induced Skeletal Muscle Atrophy in Male mice". Egyptian Journal of Histology, , , 2023, -. doi: 10.21608/ejh.2023.240872.1958
Rashad, H., Ibrahim, N., Ahmed, S., Abd el all, M., Sadek, N. (2023). 'Histological Study to Compare the Effect of Atomoxetine Versus Formetrol on Dexamethasone-Induced Skeletal Muscle Atrophy in Male mice', Egyptian Journal of Histology, (), pp. -. doi: 10.21608/ejh.2023.240872.1958
Rashad, H., Ibrahim, N., Ahmed, S., Abd el all, M., Sadek, N. Histological Study to Compare the Effect of Atomoxetine Versus Formetrol on Dexamethasone-Induced Skeletal Muscle Atrophy in Male mice. Egyptian Journal of Histology, 2023; (): -. doi: 10.21608/ejh.2023.240872.1958
Histological Study to Compare the Effect of Atomoxetine Versus Formetrol on Dexamethasone-Induced Skeletal Muscle Atrophy in Male mice
Articles in Press, Accepted Manuscript, Available Online from 20 November 2023
Histology and cell biology department, Faculty of medicine, Fayoum university
Abstract
Abstract Back ground: Atrophy of skeletal muscles is still a serious clinical problem. Formoterol, an agonist of the B2- adrenergic receptor, may prevent this atrophy. An FDA-approved inhibitor of reuptake of norepinephrine called atomoxetine was effective in the prevention of skeletal muscle atrophy. Aim of work: Compare the effect of atomoxetine versus formetrol on dexamethasone-induced skeletal muscle atrophy in male mice. Material and methods: Forty-eight adult male albino mice were divided into six groups (8 mice each): Group 1 (control group) animals were injected intraperitoneally with 0.5ml sterile saline daily for seven days. Group 2 (dexamethasone treated group) animals were injected intraperitoneally with 10mg/kg/day dexamethasone for seven days to induce muscle atrophy. Group 3 (atomoxetine only treated group): animals received atomoxetine at a dose of 6mg/kg/day orally using insulin syringe without needle for seven days. Group 4 (atomoxetine + dexamethasone treated group): animals received both dexamethasone and atomoxetine at same doses and routes of administrationin as groups 2 and 3 respectively. Group 5 (formertrol only treated group): animals were injected intraperitoneally with 0.6 mg/kg/day formetrol for seven days. Group 6 (formertrol + dexamethasone treated group): animals received both dexamethasone and formetrol at same doses and routes of administration as groups 2 and 5 respectively. Sections were stained with hematoxylin and eosin stain & Picro Sirius red (PSR) histochemical reaction. Immunohistochemical staining was done using nuclear factor kappa-B (NF-κB) and heat shock protein (Hsp70). Area percent of collagen fibers deposition, area percent of nuclear factor kappa-B immunoexpression, area percent of heat shock protein 70 immunoexpression and diameter of muscle fiber were measured. Results: Group 4 (atomoxetine and dexamethasone treated group) and Group 6 (formertrol and dexamethasone treated group) showed increase in diameter of muscle fibers as compared to dexamethasone group. Conclusion: Formetrol has a potential role in preventing skeletal muscle atrophy.