MiRNA-146a in Mesenchymal Stem Cells Exosome Alleviates Histological Structure of Pulmonary Tissue Via IRAK1 / NF-κB Pathway in Experimentally Induced Bronchial Asthma

Document Type : Original Article

Authors

1 Department of Histology and Cell Biology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.

2 Department of Histology and Cell Biology, Faculty of Medicine, Zagazig University, Egypt

Abstract

Introduction: Bronchial asthma is a multifaceted respiratory disorder that represents a significant global public health concern, afflicting millions of individuals and resulting in considerable morbidity and mortality.
Aim of Work: To unravel the intricate ultra-structural and cyto-molecular potential impacts of exosomes released by mesenchymal stem cells (MSCs) as biocompatible nanocarriers of miR-146 on experimentally induced bronchial asthma.
Materials and Methods: Thirty-two male adult albino rats were splitted into three groups. Group I (control group), group II (experimentally induced asthma group): Every rat got 25 μg house dust mite (HDM) extract suspended in 35 μL of saline for five sequential days weekly for four weeks intranasally, and group III (exosome-treated groups): rats were administered HDM as group II, and after two weeks, they received 100 μg MSC-derived exosomes in 0.5 ml PBS intravenous through the tail vein, once/day for two weeks. Pulmonary tissue samples were examined for histopathological, ultrastructural, and NF-κB immune expression beside RT-PCR estimation of miR-146a, IRAK1, and NF-κB.
Results: Observable morphological and ultrastructural restoration of pulmonary tissue architecture in the MSCs exosomes-treated group compared to induced asthma group were recorded. Furthermore, the significant suppression of IRAK1 and NF-κB, along with the expantion of miR-146a and reduced immunohistochemical representation of NF-κB in the exosomes-treated group, indicated the potential molecular mechanisms for the observed histo-morphometric impacts.
Conclusion: Our study outlined the therapeutic implicit of miRNA-146 in mesenchymal stem cell exosomes via IRAK1/NF-κB pathway as a novel and effective approach for managing bronchial asthma and paving the way for further research.

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