Mohamed, E., Samak, M. (2024). MiRNA-146a in Mesenchymal Stem Cells Exosome Alleviates Histological Structure of Pulmonary Tissue Via IRAK1 / NF-κB Pathway in Experimentally Induced Bronchial Asthma. Egyptian Journal of Histology, 47(4), 1354-1367. doi: 10.21608/ejh.2023.235299.1947
Eman M Mohamed; Mai Samak. "MiRNA-146a in Mesenchymal Stem Cells Exosome Alleviates Histological Structure of Pulmonary Tissue Via IRAK1 / NF-κB Pathway in Experimentally Induced Bronchial Asthma". Egyptian Journal of Histology, 47, 4, 2024, 1354-1367. doi: 10.21608/ejh.2023.235299.1947
Mohamed, E., Samak, M. (2024). 'MiRNA-146a in Mesenchymal Stem Cells Exosome Alleviates Histological Structure of Pulmonary Tissue Via IRAK1 / NF-κB Pathway in Experimentally Induced Bronchial Asthma', Egyptian Journal of Histology, 47(4), pp. 1354-1367. doi: 10.21608/ejh.2023.235299.1947
Mohamed, E., Samak, M. MiRNA-146a in Mesenchymal Stem Cells Exosome Alleviates Histological Structure of Pulmonary Tissue Via IRAK1 / NF-κB Pathway in Experimentally Induced Bronchial Asthma. Egyptian Journal of Histology, 2024; 47(4): 1354-1367. doi: 10.21608/ejh.2023.235299.1947
MiRNA-146a in Mesenchymal Stem Cells Exosome Alleviates Histological Structure of Pulmonary Tissue Via IRAK1 / NF-κB Pathway in Experimentally Induced Bronchial Asthma
1Department of Histology and Cell Biology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
2Department of Histology and Cell Biology, Faculty of Medicine, Zagazig University, Egypt
Abstract
Introduction: Bronchial asthma is a multifaceted respiratory disorder that represents a significant global public health concern, afflicting millions of individuals and resulting in considerable morbidity and mortality. Aim of Work: To unravel the intricate ultra-structural and cyto-molecular potential impacts of exosomes released by mesenchymal stem cells (MSCs) as biocompatible nanocarriers of miR-146 on experimentally induced bronchial asthma. Materials and Methods: Thirty-two male adult albino rats were splitted into three groups. Group I (control group), group II (experimentally induced asthma group): Every rat got 25 μg house dust mite (HDM) extract suspended in 35 μL of saline for five sequential days weekly for four weeks intranasally, and group III (exosome-treated groups): rats were administered HDM as group II, and after two weeks, they received 100 μg MSC-derived exosomes in 0.5 ml PBS intravenous through the tail vein, once/day for two weeks. Pulmonary tissue samples were examined for histopathological, ultrastructural, and NF-κB immune expression beside RT-PCR estimation of miR-146a, IRAK1, and NF-κB. Results: Observable morphological and ultrastructural restoration of pulmonary tissue architecture in the MSCs exosomes-treated group compared to induced asthma group were recorded. Furthermore, the significant suppression of IRAK1 and NF-κB, along with the expantion of miR-146a and reduced immunohistochemical representation of NF-κB in the exosomes-treated group, indicated the potential molecular mechanisms for the observed histo-morphometric impacts. Conclusion: Our study outlined the therapeutic implicit of miRNA-146 in mesenchymal stem cell exosomes via IRAK1/NF-κB pathway as a novel and effective approach for managing bronchial asthma and paving the way for further research.
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