THE POSSIBLE PROTECTIVE EFFECT OF ALFACALCIDOL ON CISPLATIN INDUCED NEPHROTOXICITY IN ADULT MALE ALBINO RATS. A HISTOLOGICAL AND IMMUNOHISTOCHEMICAL STUDY

Articles in Press

Document Type : Original Article

Authors

1 Human anatomy and embryology, faculty of medicine, Alexandria university

2 Anatomy and Embryology Department, Faculty of Medicine, Alexandria University

3 Department of Anatomy and Embryology, Faculty of Medicine, Alexandria University, Alexandria, Egypt

Abstract

Abstract:
Background: Cisplatin is an antineoplastic drug for a variety of solid-organ tumors. However, its clinical applications are restricted due to its nephrotoxicity.
Objective: To investigate the protective role of alfacalcidol against cisplatin induced renal injury.
Materials and methods: Thirty adult male albino rats, each of 180-220 gm average weight were randomly divided into three groups. Control group (Group I) divided into two sub-groups (group Ia: received standard diet and free access to water and group Ib: received sodium chloride 0.9% (6.5 ml/kg body weight) once a week for 3 times. They will be sacrificed on day 21. Cisplatin group received a dose of 6.5 mg/kg intraperitoneal (Group II) that divided into 2 subgroups (group IIa ‘‘acute model’’ received cisplatin on day 0 and sacrificed on day 5) (group IIb ‘‘chronic model’’ received cisplatin on day 0, 7, 14 and sacrificed on day 21). Alfacalcidol group received 50 ng/kg/day orally by orogastric tube starting 5 days before first dose of cisplatin till the day of sacrification (Group III). This group is also divided into acute model group (group IIIa) and chronic model group (group IIIb). Group IIIa received cisplatin on day 0 and alfacalcidol 5 days before and 5 days after cisplatin injection. They will be sacrificed on day 5. Group IIIb received cisplatin on day 0, 7, 14 and alfacalcidol orally starting 5 days before first dose of cisplatin till day 21. They will be sacrificed on day 21.
Results: cisplatin treated acute and chronic models caused mild and severe glomerular atrophy and necrosis of some epithelial cells of tubules respectively. Concomitant treatment with alfacalcidol showed a significant level of protection and restoration of normal renal structure.
Conclusion According to this study, cisplatin induces marked degenerative changes on the kidneys, which are ameliorated by alfacalcidol.

Keywords

Egyptian Journal of Histology

Articles in Press, Accepted Manuscript
Available Online from 17 July 2023

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