mohamady, R., zaky, M., Shenouda, H., Abd_elmonem, S. (2024). The Possible Protective Role of Melatonin and Exosomes Derived from Mesenchymal Stem Cells on Cisplatin Induced Testicular Injury in Adult Male Albino Rats: Histological and Immunohistochemical Study. Egyptian Journal of Histology, 47(3), 978-990. doi: 10.21608/ejh.2023.214373.1902
rania ebrahim mohamady; mohamed magdy zaky; helpies delwar Shenouda; Sahar N. Abd_elmonem. "The Possible Protective Role of Melatonin and Exosomes Derived from Mesenchymal Stem Cells on Cisplatin Induced Testicular Injury in Adult Male Albino Rats: Histological and Immunohistochemical Study". Egyptian Journal of Histology, 47, 3, 2024, 978-990. doi: 10.21608/ejh.2023.214373.1902
mohamady, R., zaky, M., Shenouda, H., Abd_elmonem, S. (2024). 'The Possible Protective Role of Melatonin and Exosomes Derived from Mesenchymal Stem Cells on Cisplatin Induced Testicular Injury in Adult Male Albino Rats: Histological and Immunohistochemical Study', Egyptian Journal of Histology, 47(3), pp. 978-990. doi: 10.21608/ejh.2023.214373.1902
mohamady, R., zaky, M., Shenouda, H., Abd_elmonem, S. The Possible Protective Role of Melatonin and Exosomes Derived from Mesenchymal Stem Cells on Cisplatin Induced Testicular Injury in Adult Male Albino Rats: Histological and Immunohistochemical Study. Egyptian Journal of Histology, 2024; 47(3): 978-990. doi: 10.21608/ejh.2023.214373.1902
The Possible Protective Role of Melatonin and Exosomes Derived from Mesenchymal Stem Cells on Cisplatin Induced Testicular Injury in Adult Male Albino Rats: Histological and Immunohistochemical Study
3Department of Histology and Cell Biology faculty of Medicine - Banha University
Abstract
Overveiw: Cisplatin (CIS) is a perfect antineoplastic medicine resulting in severe testicular toxicity. Melatonin is a potent antioxidant that can prevent gonadal toxicity. exosomes have been demonstrated to have a vital effect in tissue regeneration. Aim to the Work: To evaluate the potential protective effects of melatonin and exosomes developed from bone marrow mesenchymal stem cells (BMSC-EX) in testicular toxicity caused by cisplatin. Materials and Methods: Fifty rats were divided in a random way into five equal groups. Group I (control group). Group II were given a single dose (8 mg/kg) of cisplatin intraperitoneally I.P. Group III (melatonin group): intra peritoneal administration of melatonin (4 ɱg/kg) started 5 days before cisplatin injection for 15 days. Group IV (exosomes group): a single intra venous dose of 100 μց BMSC-EX one day before cisplatin dose. Group V (melatonin and exosomes). Specimens of the testicles were collected and processed for immunohistochemical and histological analysis. Results: Group II declared distortion, irregular outline of the seminiferous tubule, with critical accumulation (P <0.01) of collagen fibers. A substantial elevation was present (P <0.01) in TNF-α immunostaining and substantial reduction (P <0.01) was seen in AR and PCNA immunostaining in comparison to control group. Groups III and IV exhibited some microscopic histological variations development, a critical reduction (P <0.01) was also observed in collagen fibers deposition and TNF-α immunostaining and critical elevation (P <0.01) was present in AR and PCNA immunostaining in comparison to group II. In comparison to control group, group V histological structure was similar. Conclusion: Each of melatonin and exosomes can protect against testicular toxicity caused by cisplatin when administered before and during cisplatin therapy, but their combined administration has better results.
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