The Possible Regenerative Capability of Harmine on Diabetic Pancreas Experimentally Induced by Streptozotocin in Adult Male Rats

Document Type : Original Article

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1 histology

2 histology, faculty of medicine, benha university

Abstract

Introduction: A significant global public health issue is diabetes. By repairing, maintaining, or increasing the original tissue function, regenerative medicine aims to lessen the patients' suffering. Due to their ability to multitask, herbal medicines are gaining attractiveness in traditional medicine.
Aim to the Work: To study the possible regenerative capacity of Harmine on diabetic pancreas experimentally induced by streptozotocin in adult male rats.
Material and Methods: Thirty adult albino rats divided equally into three groups. Control group (group I). Diabetic group (group II): rats were nurtured a fat excessive diet pro three weeks prior to a single intraperitoneal dose of freshly supplied STZ (40 mg/kg). Diabetic+ harmine group (group III): rats treated as group II and on day eight after the onset of diabetes, harmine was given in a dose 6.5 mg/kg over the course 28 daylights in a gavage approach. Paraffin sections were practiced for H&E and Immunohistochemical study.
Results: The pancreatic islets histology of the diabetic group concealed marked distortion alongside cellular vacoulations and nuclear pyknosis. Consequently confirmed immunohistochemically by significant increase (p>0.01) in caspase-3 and significant decrease (p>0.01) in insulin, Ki67 and HSP70 immunoexpression. The pancreatic islets of diabetic rats given harmine for four weeks displayed reduced degenerative structure, greater islets mass, and fewer vacuolated cells. Immunohistochemical beta cell indicators, such as a statistically significant decrease (p>0.01) in caspase-3 immunoexpression and a significant increase (p>0.01) in insulin, Ki67, and HSP70 immunoexpression compared to group II , served as proof of this.
Conclusion: Harmine is proficient pro beta cell projiferation, expansion of islet build and recover glycemic check. These findings implying harmine analogs possibly will partake unique therapeutic potential for the medication of human diabetes.

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