Comparative between the Effect of Bone Marrow-Derived Mesenchymal Stem Cells and Mobilized Bone Marrow-Derived Stem Cells on Experimentally-Induced Acute Kidney Injury in Adult Male Albino Rat (Histological, Immunohistochemical and Biochemical Study)

Document Type : Original Article

Authors

1 histology and cell biology, medicine,tanta university, samanoud, Egypt

2 Histology faculty of medicine Tanta university, Tanta, Egypt

3 histology, faculty of medicine, tanta university, El Mansoura, Egypt

4 histology, faculty of Medicine, Tanta University, Cairo, Egypt

5 histology, Faculty of medicine, Tanta University

Abstract

Introduction : Cisplatin was used as a model of Acute Kidney Injury (AKI). Bone marrow-derived mesenchymal stem cells (BM-MSCs) were multipotent and capable of self-renewal. Granulocyte-colony stimulating factor (G-CSF) can move hematopoietic cells from the bone marrow into the circulation having therapeutic value in improving AKI.
Aim of the Work: Comparison between the effect of bone marrow-derived mesenchymal stem cells and mobilized bone marrow-derived stem cells on experimentally-induced acute kidney injury in adult male albino rat renal cortex using histological and immunohistochemical methods.
Materials and Methods: Five main groups of 42 adult male albino rats were established; Group 0; the source of BM-MSCs. Group I; Control including two subgroups IA (kept without treatment) & subgroup IB (animals received single dose of 0.5 ml of media into tail’s vein). Group II: animals were injected intraperitoneally with cisplatin once in a dose of 10 mg/kg for induction of AKI. Group III: animals were injected by cisplatin as in group II and treated by BM-MSCs suspension 0.5 ml in a single dose into the rat tail vein 24 h after injection. Group IV: animals were injected by cisplatin as in group II and treated by 100 μgm / kg G-CSF subcutaneously every day for 5 days 24 h after injection. Blood samples were collected to measure blood urea nitrogen and serum creatinine. Animals were sacrificed at the 7th day of experiment except group II after 24 hours. Kidneys were dissected out to obtain renal cortex, processed for histological studies. Histological grading for the degree of AKI, statistical and biochemical analysis were done.
Results: BM-MSCs treated group III exhibited mild improvement while G-CSF treated group IV showed marked improvement in histological structure of renal cortex and renal functions.
Conclusion: G-CSF was more beneficial than BM-MSCs in alleviating cisplatin induced AKI.

Keywords

Egyptian Journal of Histology
Volume 46, Issue 4
December 2023
Pages 1545-1562

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