Document Type : Original Article
Authors
1
oral biology, faculty of dentistry, Cairo University, Cairo
2
Professor of Oral Biology, Oral Biology Department, Faculty of Dentistry, Cairo University, Cairo, Egypt.
3
Lecturer of Oral Biology, Faculty of Dentistry, Cairo University, Cairo, Egypt.
4
Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.
5
PhD, Associate Professor, Oral Biology department, Faculty of Dentistry, Cairo University, 11 Al Saraya, Al Manial, Giza Governorate, 11553 Egypt
Abstract
Introduction: Diabetes mellitus is a serious disease of worldwide concern. Therapeutic bee venom (BV) and bone marrow mesenchymal stem cells (BMSCs) were widely applied in various diseases.
Aim of the Work: This study was conducted to compare the effect of BV and BMSCs on the submandibular salivary glands of streptozotocin (STZ)-induced diabetic rats.
Materials and Methods: In the ongoing study, 40 male rats were used. Five rats were utilized for BMSCs isolation and culture, 5 rats served as the control group, however the remaining 30 rats were distributed as follow: untreated diabetic group; received a single intraperitoneal (IP) dose of STZ, BV treated diabetic group; diabetic rats received a daily IP dose of 0.5 mg/kg BV for 4 weeks, and BMSCs treated diabetic group; diabetic rats received a single IV injection of BMSCs. All animals were euthanized after a month, the submandibular salivary glands were dissected and evaluated by histological, immunohistochemical and quantitative Real-Time Polymerase Chain Reaction (qRT- PCR) examinations. Finally, all obtained data were statistically analyzed.
Results: Regarding control group the salivary gland architecture was normal, while the diabetic group revealed degenerative glandular changes. Both diabetic treated groups showed improved histological pictures, however; improvement was more obvious in the BMSCs treated group in comparison to the BV treated one. Regarding the diabetic group, a significantly increased inducible nitric oxide synthase
(iNOS) immunoexpression and Hemeoxygenase-1 (HO-1) expression were noticed. However, following BV and BMSCs treatments, a significantly decreased iNOS immunoreactivity and overexpression of HO-1 gene were noticed.
Conclusion: Both BMSCs and BV treatments ameliorated degenerative effects of diabetes, yet; BMSCs exhibited a more significant therapeutic effect than BV.
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