Effect of Abrupt and Gradual withdrawal of Acrylamide on Acrylamide Induced Hepatic and Renal Cortical Injury of Adult Male Albino Rats: Histological and Immunohistochemical Study

Ahmed, Amira; Ibrahim, Randa Ahmed; Ali, Azza; Khairy, Esraa Mohammed

doi:10.21608/ejh.2022.115268.1634

Effect of Abrupt and Gradual withdrawal of Acrylamide on Acrylamide Induced Hepatic and Renal Cortical Injury of Adult Male Albino Rats: Histological and Immunohistochemical Study

Document Type : Original Article

Authors

¹ Histology and Cell Biology Department, Faculty of Medicine, El-Minia University, Minia,Egypt

² Faculty of medicine - Minia university histology and cell biology department, Minia,Egypt

³ histology and cell biology, faculty of medicine,Minia university

⁴ histology and cell biology department, faculty of medicine, Minia university, Minia, Egypt

10.21608/ejh.2022.115268.1634

Abstract

Introduction: Acrylamide (AA) became an urgent public health interest as it is commonly consumed in fast food cooked at high temperatures. Various health organizations recommend stoppage of its use in certain food due to its health hazards.
Aim of the Work: To investigate the effect of abrupt and gradual withdrawal of acrylamide on its destructive impact on the hepatic and renal cortical tissues.
Materials and Methods: Rats were classified into 4 groups 10 rats in every group. The control group (group I). The acrylamide group (group II): Acrylamide was orally administered at the dose of 20 mg/kg/day for 3 weeks. The abrupt withdrawal group (group III): Acrylamide was given by the same route as group II for 3 weeks, then abruptly stopped in the following 3 weeks. The gradual withdrawal group (group IV): Acrylamide was given by the same route as group II for 3 weeks then the acrylamide was gradually withdrawn in the following three weeks. Liver and renal cortical tissues were taken and used for histological (H&E, PAS and Masson stain), immunohistochemical (PCNA, caspase 3 and alpha SMA) and morphometric studies.
Results: The histological examination of the liver and renal cortical tissue section of groups II and IV revealed marked destruction of their architecture. On the other hand, group III showed marked improvement.
Conclusion: The usage of AA caused deleterious effects in liver and renal cortical tissues mainly due to reactive oxygen species (ROS) production. Stopping AA administration showed marked improvement in the histology of liver and renal tissue if compare with the its gradual withdrawal.

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