Toxic Effect of Subchronic Use of Arsenic on the Liver of Adult Male Albino Rats and the Ameliorating Effect of Cerium Oxide Nanoparticles: Biochemical and Histological Study

Document Type : Original Article

Authors

1 Department of Human Anatomy and Embryology faculty of medicine ALEXANDRIA university, MUTAH University, JORDAN

2 DEPARTMENT OF ANATOMY AND HISTOLOGY FACULTY OF MEDICINE, MUTAH UNIVERSITY,AL-KARAK, JORDAN DEPARTMENT OF HUMAN ANATOMY AND EMBRYOLOGY, FACULTY OF MEDICINE, ZAGAZIG UNIVERSITY

3 Anatomy department,faculty of medicine.alexandria university

Abstract

Introduction: Arsenic is a heavy metal which is commonly present in the atmosphere. Most contamination of inorganic arsenic is caused by food, particularly seafood. The respiratory, cardiovascular, skin, central nervous system, kidneys ,and liver are impaired by prolonged human exposure to arsenic. Cerium oxide nanoparticles (CeONPs) has promising antioxidant regenerative and hepatoprotective potentials against various pathologies caused by oxidative stess.
Material and Methods: All rats were subdivided into 3 groups (n=10) for a period of 8 weeks study: • Group I (the control group) where rats were injected 250 μl of phosphate buffered saline (PBS) intraperitoneally every other day.• Group II (the arsenic group) received arsenic trioxide, 3mg/kg body weight/day which is equivalent to 75% of LD50 value of a 70 kg body weight human (1–4mg/kg) and lesser than one thirteenth of LD50 value of rats (40mg/kg) by orogastric tube once daily for 8 weeks.
Results: Histological and ultrastructural tests have measured the hepatic toxicity. Additionally, it is tested by determination of an oxidative stress marker. Not only were CeONPs proven safe at the proposed dosage, but they also managed to reduce hepatic injury on both histological and biochemical aspects through their concomitant administration of Arsenic. Hepatic provocation on both histological and biochemical levels appeared to be mitigated. These hepatoprotective activity has been attributed to CeONPs' observed antioxidant action as demonstrated by the large difference in rates of MDA.
Conclousion: Therefore the purpose of this analysis was to evaluate either the CeONPs Shows hepatoprotective properties whether administered to or not concurrently with Arsenic.

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