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Nasser, M., Abdullah, M., Ayad, M., El Deftar, M. (2021). Reconstruction Of Mandibular Segmental Defect Using Bone marrow Stem Cells (BMSCs) - Seeded Chitosan/Bioglass Scaffold. Egyptian Journal of Histology, (), -. doi: 10.21608/ejh.2021.86833.1533
Mahmoud ELGHARIB Nasser; Mahmoud Abdullah; Mohamed Ayad; Mervat ELGHARIB El Deftar. "Reconstruction Of Mandibular Segmental Defect Using Bone marrow Stem Cells (BMSCs) - Seeded Chitosan/Bioglass Scaffold". Egyptian Journal of Histology, , , 2021, -. doi: 10.21608/ejh.2021.86833.1533
Nasser, M., Abdullah, M., Ayad, M., El Deftar, M. (2021). 'Reconstruction Of Mandibular Segmental Defect Using Bone marrow Stem Cells (BMSCs) - Seeded Chitosan/Bioglass Scaffold', Egyptian Journal of Histology, (), pp. -. doi: 10.21608/ejh.2021.86833.1533
Nasser, M., Abdullah, M., Ayad, M., El Deftar, M. Reconstruction Of Mandibular Segmental Defect Using Bone marrow Stem Cells (BMSCs) - Seeded Chitosan/Bioglass Scaffold. Egyptian Journal of Histology, 2021; (): -. doi: 10.21608/ejh.2021.86833.1533

Reconstruction Of Mandibular Segmental Defect Using Bone marrow Stem Cells (BMSCs) - Seeded Chitosan/Bioglass Scaffold

Articles in Press, Accepted Manuscript, Available Online from 16 September 2021  XML
Document Type: Original Article
DOI: 10.21608/ejh.2021.86833.1533
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Authors
Mahmoud ELGHARIB Nasser email 1; Mahmoud Abdullah2; Mohamed Ayad3; Mervat ELGHARIB El Deftar4
1oral surgery
2Oral surgery, Faculty Of Dentistry, Azhar University. Cairo, Egypt
3Veterinary, Faculty of Veterinary Medicine, Cairo University, Egypt
4Tissue Culture and Cytogenetics, National cancer institute, Cairo, Egypt
Abstract
Abstract:
Objectives: To reconstruct a critically sized segmental mandibular bony defect using chitosan/ bioactive glass scaffold seeded with osteogenically differentiated bone marrow mesenchymal stem cells with the evaluation of the engineered bone tissue. Materials and methods: 18 adult male mongrel dogs were selected randomly to be used in the present study. The experimental protocol was divided in to five stages as follow; A. Extraction of lower first molar. B. Bone marrow aspiration from the tibias of the selected dogs. C. Bone marrow mesenchymal stem cells (BMMSCs) isolation and cell culturing followed by osteogenic differentiation of the cells. D. Bioactive glass/ Chitosan scaffold preparation and seeding of BMSCs. E. Mandibular segmental defect creation followed by implantation of the engineered construct into the defect site. F. Evaluation of the newly formed osseous tissue via radiographic density measurements, histological examination and histomorphometric analysis. Results: Radiographic evaluation of the constructed bone tissue revealed that the highest bone density (259.23 +/- 52.42 %) was recorded by group group I2 after 16 weeks. H& E stained engineered bone showed various stages of bone development where the newly formed bone was integrated with the host bone. Histomorphometric analysis revealed the increase in areas of mature newly formed bone by increasing time intervals. The highest mean bone area fraction value (47.72+/-1.94%) was recorded by group I2 at 16week time interval. Conclusion: Under the circumstances of the present study we could suggest that chitosan/ bioglass tissue engineering scaffolds seeded with BMSCs could be a reliable treatment modality for reconstruction of critically-sized bone defects.
Keywords
Chitosan; Bioglass; Bone marrow stem cells; Segmental defect
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