Abd-Elhafez, A., Elkelany, M., Mousa, A., El-deeb, T., Kassab, A. (2022). The Possible Curative Role of Bone Marrow-Derived Mesenchymal Stem Cells in Bleomycin-Induced Skin Changes in Adult Male Albino Rats. Egyptian Journal of Histology, 45(4), 1125-1145. doi: 10.21608/ejh.2021.80788.1505
Amal Abd-Elhafez; Maram Elkelany; Amany Mousa; Thorya El-deeb; Amira Adly Kassab. "The Possible Curative Role of Bone Marrow-Derived Mesenchymal Stem Cells in Bleomycin-Induced Skin Changes in Adult Male Albino Rats". Egyptian Journal of Histology, 45, 4, 2022, 1125-1145. doi: 10.21608/ejh.2021.80788.1505
Abd-Elhafez, A., Elkelany, M., Mousa, A., El-deeb, T., Kassab, A. (2022). 'The Possible Curative Role of Bone Marrow-Derived Mesenchymal Stem Cells in Bleomycin-Induced Skin Changes in Adult Male Albino Rats', Egyptian Journal of Histology, 45(4), pp. 1125-1145. doi: 10.21608/ejh.2021.80788.1505
Abd-Elhafez, A., Elkelany, M., Mousa, A., El-deeb, T., Kassab, A. The Possible Curative Role of Bone Marrow-Derived Mesenchymal Stem Cells in Bleomycin-Induced Skin Changes in Adult Male Albino Rats. Egyptian Journal of Histology, 2022; 45(4): 1125-1145. doi: 10.21608/ejh.2021.80788.1505
The Possible Curative Role of Bone Marrow-Derived Mesenchymal Stem Cells in Bleomycin-Induced Skin Changes in Adult Male Albino Rats
1Histology Department, Faculty of Medicine, Tanta University
2Histology Department Faculty of Medicine Tanta University
3Histology Department,Faculty of Medicine Tanta University
4Histology and Cell Biology Department, Faculty of Medicine,Tanta University, Egypt
Abstract
Introduction: Bleomycin is a chemotherapeutic agent that has been applied in the treatment of many tumors. Its application results in various cutaneous side effects. The role of bone marrow-derived mesenchymal stem cells [BM-MSCs] has been proved in tissue regeneration. Aim of the Work: Study of the effect of bleomycin on the histological structure of the skin of adult male albino rats and evaluation of the possible curative role of BM-MSCs. Materials and Methods: Forty-five adult male albino rats were divided into two groups. Control group included 15 rats. Experimental group included 30 rats which were subdivided into three equal subgroups: 2A, 2B & 2C. Rats of the experimental group were injected with bleomycin subcutaneously for four weeks. Each rat of subgroup 2A received [0.1mg] of bleomycin. Each rat of subgroup 2B received [1ml] of the culture media of the bone marrow cells one day after the last bleomycin dose. Each rat of subgroup 2C received BM-MSCs (2.5×106) one day after the last bleomycin dose. Skin specimens were examined by the light and electron microscopy. Immunohistochemical study was done by using antibodies to transforming growth factor beta (TGF-β). Evaluation of the collagen surface area percentage and the number of TGF-β positive cells was performed. Results: Experimental subgroups 2A & 2B showed the disturbance of the normal architecture of the skin. There was a significant increase in the surface area percentage of collagen and number of TGF-β positive cells. Ultrastructurally, some keratinocytes showed cytoplasmic vacuolation, swollen disrupted mitochondria and nuclear changes. In contrast, the experimental subgroup 2C showed minimal structural changes and a non-significant difference in all mentioned parameters. Conclusion: Bleomycin altered the normal structure of the skin of adult male albino rats. Bone marrow-derived MSC improved the skin changes induced by bleomycin.
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