The Possible Role of Orthoboric Acid on Adipogenesis in Adult Male Albino Rat. Histological and Immunohistochemical Study

Document Type : Original Article

Authors

1 Histology department, Faculty of Medicine, Ain Shams University, Cairo, Egypt

2 Histology and cell biology. Faculty of Medicine, Ain Shams University

3 Histology Faculty of Medicine Ain Shams University

4 Histology department, Faculty of Medicine Ain Shams University

Abstract

Background: Obesity is a worldwide problem which leads to increased morbidity and rate of deaths. Clinical use of some therapeutic options for the prevention of overweightness is limited due to their dangerous side effects. Orthoboric acid is a new safe and beneficial treatment for prevention of high fat diet induced obesity.
Aim: Study the effect of orthoboric acid on adipogenesis in rats.
Materials and Methods: The study included 30 adult male albino rats. The rats were divided into 4 main groups. Group I: control. Group II (High Fat Diet ′′HFD′′ group): IIa (HFD 8 weeks), IIb (HFD 12 weeks). Group III (HFD and orthoboric acid group) for 8 weeks. GroupIV (HFD for 12 weeks but starting from the 8th week the rats were given orthoboric acid daily for 4 weeks). Orthoboric acid was used at dose of 2.5 mg/rat given orally via intragastric tube. Body weights of all rats were recorded weekly during the experiment. Adipose tissues pecimens were collected at the end of experiment and processed for H&E, oil red O stain and antiβ - catenin immunohistochemistry. Morphometric and statistical analysis were also done.
Results: HFD group showed significant increase in body weight and in size of adipocytes compared to the control group meanwhile, groups of orthoboric acid showed a significant decrease in body weight and size of adipocytes relative to HFD group. As regard effect of orthoboric acid on adipogenesis, groups treated with orthoboric acid showed positive immunohistochemical reaction for β- catenin with subsequent decrease in lipid accumulation confirmed with less optically dense oil red O staining compared to HFD group.
Conclusions: Low dose of oral orthoboric acid was able to reduce body weight. Therefore, it can be considered as a suitable treatment for obesity due to its vital role in inhibition of adipogenesis

Keywords

Egyptian Journal of Histology
Volume 45, Issue 1
March 2022
Pages 180-191

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