Mansy, M., Soliman, M., Mubarak, R., Shamel, M. (2021). The Effect of EGF on VEGF Expression on Submandibular Salivary Gland of Albino Rats Receiving Doxorubicin. Egyptian Journal of Histology, 44(2), 418-424. doi: 10.21608/ejh.2020.31011.1299
Mohamed Mansy; Malak Soliman; Rabab Mubarak; Mohamed Shamel. "The Effect of EGF on VEGF Expression on Submandibular Salivary Gland of Albino Rats Receiving Doxorubicin". Egyptian Journal of Histology, 44, 2, 2021, 418-424. doi: 10.21608/ejh.2020.31011.1299
Mansy, M., Soliman, M., Mubarak, R., Shamel, M. (2021). 'The Effect of EGF on VEGF Expression on Submandibular Salivary Gland of Albino Rats Receiving Doxorubicin', Egyptian Journal of Histology, 44(2), pp. 418-424. doi: 10.21608/ejh.2020.31011.1299
Mansy, M., Soliman, M., Mubarak, R., Shamel, M. The Effect of EGF on VEGF Expression on Submandibular Salivary Gland of Albino Rats Receiving Doxorubicin. Egyptian Journal of Histology, 2021; 44(2): 418-424. doi: 10.21608/ejh.2020.31011.1299
The Effect of EGF on VEGF Expression on Submandibular Salivary Gland of Albino Rats Receiving Doxorubicin
2Oral Biology, Faculty of Dentistry, Cairo University
3Oral Biology department, Faculty of Dentistry, The British University in Egypt.
Abstract
Background: Vascular endothelial growth factor (VEGF) has two prominent biological effects of inducing mitogenesis and migration of vascular endothelial cells and to produce permeability on the microvasculature. Epidermal growth factor (EGF), known by its mitogenic activity on different types of cells, could induce the proliferation and differentiation of the epithelial cells and stimulate its regeneration. Doxorubicin (DXR) is a widely used anticancer drug and is one of most commonly used chemotherapeutic drugs, however it may cause some serious complications such as toxicity in various tissues in the body. Aims: The present study aims to investigate the effect of EGF on the VEGF immunoexpression in submandibular salivary gland tissue of rats receiving Doxorubicin (DXR). Methods and Material: 30 male Albino rats, two months old and weighing 200-250 gm body weight, were divided equally into three groups as follows: Group I (control group). Group II: rats received 20 mg/kg body weight DXR as a single intra peritoneal injection. Whereas, group III: rats received the same dose of DXR and on the next day they were injected intraperitoneally with EGF in a daily dose of 10μg/kg body weight for 7 consecutive days. Submandibular salivary glands were dissected out and processed for histological investigation and immunohistochemical localization of VEGF in the glandular tissue. Statistical analysis used: ANOVA was used to compare the mean area percentage of VEGF immunostaining among the specimens of different groups followed by Tukey’s Multiple Comparison Test. Results: VEGF expression in submandibular salivary glands of rats significantly decreased after DXR injection. However, daily intraperitoneal injections of EGF restored normal levels of VEGF expression in the glandular tissue. Conclusions: EGF preserved glandular architecture after DXR injection and consequently the immunohistochemical expression of VEGF in the glands to approximately the normal level.
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