• Home
  • Browse
    • Current Issue
    • By Issue
    • By Author
    • By Subject
    • Author Index
    • Keyword Index
  • Journal Info
    • About Journal
    • Aims and Scope
    • Editorial Board
    • Publication Ethics
    • Indexing and Abstracting
    • Peer Review Process
  • Guide for Authors
  • Submit Manuscript
  • Previous Issues
  • Contact Us
 
  • Login
  • Register
Home Articles List Article Information
  • Save Records
  • |
  • Printable Version
  • |
  • Recommend
  • |
  • How to cite Export to
    RIS EndNote BibTeX APA MLA Harvard Vancouver
  • |
  • Share Share
    CiteULike Mendeley Facebook Google LinkedIn Twitter
Egyptian Journal of Histology
arrow Articles in Press
arrow Current Issue
Journal Archive
Volume Volume 48 (2025)
Volume Volume 47 (2024)
Volume Volume 46 (2023)
Volume Volume 45 (2022)
Volume Volume 44 (2021)
Volume Volume 43 (2020)
Issue Issue 4
Issue Issue 3
Issue Issue 2
Issue Issue 1
Volume Volume 42 (2019)
Volume Volume 41 (2018)
Volume Volume 40 (2017)
Aziz, J., Aidaros, A., Ali, A., Sabry, M. (2020). Ameliorating Effect of Alpha-lipoic Acid on Methotrexate-induced Histological and Biochemical Changes in The Lung of Adult Albino Rat. Egyptian Journal of Histology, 43(3), 878-890. doi: 10.21608/ejh.2019.19780.1202
Joseph Aziz; Abd-Elmawla Elsayed Aidaros; Ahmad Faraj Ali; Mohamed Amed Sabry. "Ameliorating Effect of Alpha-lipoic Acid on Methotrexate-induced Histological and Biochemical Changes in The Lung of Adult Albino Rat". Egyptian Journal of Histology, 43, 3, 2020, 878-890. doi: 10.21608/ejh.2019.19780.1202
Aziz, J., Aidaros, A., Ali, A., Sabry, M. (2020). 'Ameliorating Effect of Alpha-lipoic Acid on Methotrexate-induced Histological and Biochemical Changes in The Lung of Adult Albino Rat', Egyptian Journal of Histology, 43(3), pp. 878-890. doi: 10.21608/ejh.2019.19780.1202
Aziz, J., Aidaros, A., Ali, A., Sabry, M. Ameliorating Effect of Alpha-lipoic Acid on Methotrexate-induced Histological and Biochemical Changes in The Lung of Adult Albino Rat. Egyptian Journal of Histology, 2020; 43(3): 878-890. doi: 10.21608/ejh.2019.19780.1202

Ameliorating Effect of Alpha-lipoic Acid on Methotrexate-induced Histological and Biochemical Changes in The Lung of Adult Albino Rat

Article 15, Volume 43, Issue 3, September 2020, Page 878-890  XML PDF (3.06 MB)
Document Type: Original Article
DOI: 10.21608/ejh.2019.19780.1202
Cited by Scopus (3)
View on SCiNiTO View on SCiNiTO
Authors
Joseph Aziz email orcid 1; Abd-Elmawla Elsayed Aidaros2; Ahmad Faraj Ali3; Mohamed Amed Sabry4
1ِAnatomy and embryology department, faculty of medicine, Zagazig university
2Anatomy and embryology department, Faculty of medicine, Zagazig university
3Faculty of medicine, ALjabal Algharbi university
4Anatomy department, Faculty of medicine, Zagazig university
Abstract
Background: Owing to its antioxidant, anti-inflammatory and anti-fibrotic properties, alpha-lipoic acid (ALA) has been evaluated in a diversity of degenerative diseases and experimental toxicological studies.
Aim of the Work: This study was performed to assess the ability of ALA to reverse the histological and biochemical alterations that take place in the rat lungs after exposure to methotrexate.
Materials and Methods: This study was done using thirty-two male Wistar albino rats (180–220 g). The animals were divided into four equal groups. The control group: received distilled water by oral gavage for ten days and injected intraperitoneally (i.p.) with1 ml of physiologic saline (0.09% NaCl) solution on the fourth day. ALA group: received ALA (200 mg/kg once daily) dissolved in distilled water and given by oral gavage for ten days. Methotrexate (MTX) group: injected with a single dose of 20mg/kg MTX i.p. on the fourth day of the study. MTX+ALA group: received an oral dose of ALA (200 mg/kg) for ten days and a single dose of MTX (20mg /kg) i.p. on the fourth day. By the end of the experiment, all the animals were anaesthetized using phenobarbital 3%. Blood samples were collected to measure c-reactive protein (CRP). The lungs were dissected out and prepared for tissue homogenates, routine histological study and caspase-3 immunohistochemical evaluation.
Results: In the MTX group, there were high levels of CPR, glutathione (GSH) and malondialdehyde (MDA) with reduction of superoxide dismutase (SOD) and catalase (CAT) activities. Lung sections revealed marked inflammatory cells infiltration, thickening of interalveolar septa, congested blood vessels, intrapulmonary hemorrhages, abundant collagen fibers and significant expression of caspase-3. In ALA+MTX group, there was an overt improvement in lung histoarchitecture. Additionally, SOD activity. CRP, MDA levels and caspase-3 expression were significantly less.
Conclusion: Administration of ALA can work against oxidative damage in the lung induced by MTX.
Keywords
Alpha-lipoic acid; caspase-3; lung; methotrexate; rat
Statistics
Article View: 834
PDF Download: 1,010
Home | Glossary | News | Aims and Scope | Sitemap
Top Top

Journal Management System. Designed by NotionWave.