Mostafa, R., Sidhom, M., Zoair, M., El-Bermawy, M., elmorshedy, K. (2025). Role of Heat Shock Pretreated Bone Marrow Mesenchymal Stem Cells Versus Non-Treated Ones in Repair of Experimentally Induced Premature Ovarian Insufficiency by Doxorubicin. Egyptian Journal of Histology, 48(2), 657-672. doi: 10.21608/ejh.2024.269354.2019
Rawda Fouad Mostafa; Makram Fahmy Sidhom; Mona mohammed Zoair; Manal Ibrahim El-Bermawy; kadreya elsayed elmorshedy. "Role of Heat Shock Pretreated Bone Marrow Mesenchymal Stem Cells Versus Non-Treated Ones in Repair of Experimentally Induced Premature Ovarian Insufficiency by Doxorubicin". Egyptian Journal of Histology, 48, 2, 2025, 657-672. doi: 10.21608/ejh.2024.269354.2019
Mostafa, R., Sidhom, M., Zoair, M., El-Bermawy, M., elmorshedy, K. (2025). 'Role of Heat Shock Pretreated Bone Marrow Mesenchymal Stem Cells Versus Non-Treated Ones in Repair of Experimentally Induced Premature Ovarian Insufficiency by Doxorubicin', Egyptian Journal of Histology, 48(2), pp. 657-672. doi: 10.21608/ejh.2024.269354.2019
Mostafa, R., Sidhom, M., Zoair, M., El-Bermawy, M., elmorshedy, K. Role of Heat Shock Pretreated Bone Marrow Mesenchymal Stem Cells Versus Non-Treated Ones in Repair of Experimentally Induced Premature Ovarian Insufficiency by Doxorubicin. Egyptian Journal of Histology, 2025; 48(2): 657-672. doi: 10.21608/ejh.2024.269354.2019
Role of Heat Shock Pretreated Bone Marrow Mesenchymal Stem Cells Versus Non-Treated Ones in Repair of Experimentally Induced Premature Ovarian Insufficiency by Doxorubicin
1Anatomy and embryology. Faculty of medicine. Tanta University
2Anatomy and embryology faculty of medicine tanta university
3anatomy and embryology tanta college of medicine.egypt and king khalid university saudia arabia
Abstract
Introduction: Premature ovarian insufficiency (POI) is one of the most severe problems threatening the reproductive health of women of normal childbearing age. Aim of the Work: This study aimed to throw a light on the effect of heat shock (HS) pretreated bone marrow mesenchymal stem cells (BM- MSCs) versus non-treated ones in repair of DOX-induced POI. Material and Methods: Fifty-six adult female albino rats were utilized and divided into three groups; Stem cells donor group included 10 rats, Control Group included 16 rats, Experimental group (30 rats); each rat received (1ml) 2.1 mg/kg BW Doxorubicin (DOX) once every 3rd day for seven IP injections. Then, this group was subdivided into three subgroups (n=10 each); subgroup III-A (DOX- treated), subgroup III-B (DOX+ routine BM-MSCs) and subgroup III-C (DOX+ HS-pre-treated BM-MSCs). Green fluorescent protein (GFP) was used for labeling and tracing the injected BM- MSCs. E2 and FSH levels were measured, rats were sacrificed and ovarian specimens were possessed for histological, immuno-histochemical, morphometric studies and statistical analysis. Results: The ovarian function in rats that received heat shock pre-treated BM-MSCs revealed significant increase of the serum level of E2 and significant decrease of FSH than in rats received routine ones. Heat shock pre-treatment increased homing of BM-MSCs which was proved by the significant increase of fluorescence traced GFP-labeled BM-MSCs in subgroup III-C as compared to subgroup III-B. There was marked improvement in the histological structure of ovarian sections of the rats received heat shock pre-treated BM-MSCs more than those received routine ones with significant decrease of apoptosis evidenced by caspase-3 immunoreactivity. Conclusion: Heat shock pre-treated BM-MSCs had a great effect in reducing the degenerative changes associated with DOX induced POI than the routine ones.
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