Role of Heat Shock Pretreated Bone Marrow Mesenchymal Stem Cells Versus Non-Treated Ones in Repair of Experimentally Induced Premature Ovarian Insufficiency by Doxorubicin

Document Type : Original Article

Authors

1 Anatomy and embryology. Faculty of medicine. Tanta University

2 Anatomy and embryology faculty of medicine tanta university

3 anatomy and embryology tanta college of medicine.egypt and king khalid university saudia arabia

Abstract

Introduction: Premature ovarian insufficiency (POI) is one of the most severe problems threatening the reproductive health of women of normal childbearing age.
Aim of the Work: This study aimed to throw a light on the effect of heat shock (HS) pretreated bone marrow mesenchymal stem cells (BM- MSCs) versus non-treated ones in repair of DOX-induced POI.
Material and Methods: Fifty-six adult female albino rats were utilized and divided into three groups; Stem cells donor group included 10 rats, Control Group included 16 rats, Experimental group (30 rats); each rat received (1ml) 2.1 mg/kg BW Doxorubicin (DOX) once every 3rd day for seven IP injections. Then, this group was subdivided into three subgroups (n=10 each); subgroup III-A (DOX- treated), subgroup III-B (DOX+ routine BM-MSCs) and subgroup III-C (DOX+ HS-pre-treated BM-MSCs). Green fluorescent protein (GFP) was used for labeling and tracing the injected BM- MSCs. E2 and FSH levels were measured, rats were sacrificed and ovarian specimens were possessed for histological, immuno-histochemical, morphometric studies and statistical analysis.
Results: The ovarian function in rats that received heat shock pre-treated BM-MSCs revealed significant increase of the serum level of E2 and significant decrease of FSH than in rats received routine ones. Heat shock pre-treatment increased homing of BM-MSCs which was proved by the significant increase of fluorescence traced GFP-labeled BM-MSCs in subgroup III-C as compared to subgroup III-B. There was marked improvement in the histological structure of ovarian sections of the rats received heat shock pre-treated BM-MSCs more than those received routine ones with significant decrease of apoptosis evidenced by caspase-3 immunoreactivity.
Conclusion: Heat shock pre-treated BM-MSCs had a great effect in reducing the degenerative changes associated with DOX induced POI than the routine ones.

Keywords

Files

Share

How to cite

Statistics