Kassem, A., Elsebay, S., Mekawy, M., Fawzy, H. (2025). Comparative Histological Study on the Therapeutic Role of Bone Marrow derived Mesenchymal Stem Cells versus their Derived Exosomes on Experimentally Induced Liver Fibrosis in Adult Male Albino Rat. Egyptian Journal of Histology, 48(2), 445-458. doi: 10.21608/ejh.2025.351566.2194
Alaa Mostafa Kassem; Sara Abd El Gawad Elsebay; Mohamed Abd El Rahman Ahmed Mekawy; Heba Fawzy. "Comparative Histological Study on the Therapeutic Role of Bone Marrow derived Mesenchymal Stem Cells versus their Derived Exosomes on Experimentally Induced Liver Fibrosis in Adult Male Albino Rat". Egyptian Journal of Histology, 48, 2, 2025, 445-458. doi: 10.21608/ejh.2025.351566.2194
Kassem, A., Elsebay, S., Mekawy, M., Fawzy, H. (2025). 'Comparative Histological Study on the Therapeutic Role of Bone Marrow derived Mesenchymal Stem Cells versus their Derived Exosomes on Experimentally Induced Liver Fibrosis in Adult Male Albino Rat', Egyptian Journal of Histology, 48(2), pp. 445-458. doi: 10.21608/ejh.2025.351566.2194
Kassem, A., Elsebay, S., Mekawy, M., Fawzy, H. Comparative Histological Study on the Therapeutic Role of Bone Marrow derived Mesenchymal Stem Cells versus their Derived Exosomes on Experimentally Induced Liver Fibrosis in Adult Male Albino Rat. Egyptian Journal of Histology, 2025; 48(2): 445-458. doi: 10.21608/ejh.2025.351566.2194
Comparative Histological Study on the Therapeutic Role of Bone Marrow derived Mesenchymal Stem Cells versus their Derived Exosomes on Experimentally Induced Liver Fibrosis in Adult Male Albino Rat
1histology,faculty of medicine,ain shams university
2Histology Faculty of Medicine Ain Shams University
Abstract
Background: Liver fibrosis is a significant global health issue with limited treatment options, except for the elimination of the underlying cause or liver transplantation. Both bone marrow mesenchymal stem cells (BM-MSCs) and their exosomes (BM-MSC-EXOs) have demonstrated promise as therapeutic agents due to their ability to regenerate and immunomodulate. Aim of the Work: To compare the therapeutic role of BM-MSCs and their exosomes on the liver's structure in adult male albino rats with carbon tetrachloride-induced liver fibrosis. Material & Methods: Forty-five adult male albino rats were included in this study, 5 of them used to isolate MSCs. Group I (the control group) included ten rats. Group II included thirty rats that received CCl4 to induce liver fibrosis then subdivided equally into subgroup IIA (CCl4 group), subgroup IIB (BM-MSCs group) received BM-MSCs once and subgroup IIC (EXOs group) received EXOs once. Results: CCl4 injection significantly induced hepatic dysfunction. It caused marked histological alternations, as confirmed by H&E, electron microscopy examination, and biochemical analysis of liver function. Rats treated with BM-MSCs, and exosomes showed considerable improvement in comparison with the CCl4-treated group. There was also a significant decrease in both the area percentages of collagen and TNF-α positive hepatocytes in these groups compared to CCl4-treated group. Although the rats treated by exosomes showed more improvement in all H&E, ultrastructure, and biochemical analysis, statistically there was a non-significant difference between BM-MSCs and exosome groups. Conclusion: The study revealed that the therapeutic role of BM-MSCs-EXOs seemed to have a greater effect than the parent BM-MSCs in a rat model of liver fibrosis induced by CCl4.
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