Histological and Biochemical Comparative Study Between the Effect of Quercetin and Topiramate on Induced Diabetic Peripheral Neuropathy in Adult Male Albino Rats

Document Type : Original Article

Authors

1 Histology and Cell Biology Department, Faculty of Medicine, Beni-Suef University,

2 Histology department, Faculty of Medicine, Beni-Suef University

3 faculty of medicine benisuef university

4 Medical Histology & Cell Biology Department Faculty of Medicine-Beni-Suef University

Abstract

Introduction: Diabetic peripheral neuropathy (DPN) is a prevalent consequence for diabetes mellitus, significantly attributing to risk of amputation and mortality, if untreated.
Aim of the Work: To emphasize the possible regenerative capacities of Quercetin and Topiramate against induced DPN in rat models.
Material and Methods: 50 adult male albino rats were allocated into; control group (n=10) and experimental (diabetic) group (n=40) injected once by alloxan (150mg /kg/i.p). After establishing the diabetic model, rats were subdivided equally into 4 subgroups. Diabetic group, Quercetin treated diabetic group received Quercetin (100mg/Kg), Topiramate treated diabetic group received Topiramate (30mg/kg), and Combined treatment diabetic group received both Quercetin and Topiramate. Treatments were given by gastric gavage for 4 weeks then the blood samples were biochemically tested. Sciatic nerves were harvested for structural, ultrastructural and immunohistochemical assessment. H & E and toluidine were used for histological analyses and immunohistochemistry for assessing Caspase-3 and S-100 expressions and electron microscopy for ultrastructural examination. Data were analysed using computer software for morphometrical and statistical analysis.
Results: The diabetic group showed a remarkable structural, biochemical and immunohistochemical changes. There was a significant decline in nerve fibres count with disturbed organization, abnormal myelin configurations, axonopathy and a significant increase and decrease in Caspase-3 and S-100 immunoreactivity. Quercetin and Topiramate treatments resulted in a partial improvement with comparable effect. Interestingly, combined therapy resulted in remarkable improvement.
Conclusion: Concomitant administration of Quercetin and Topiramate have a superior therapeutic effect in a modal of a diabetic neuropathy than using either agent.

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