The Possible Therapeutic Effect of Platelet Rich Plasma Versus Platelet Rich Plasma Derived Exosomes on Experimentally Induced Ulcerative Colitis in Adult Male Albino Rats : A Histological Study

Document Type : Original Article

Authors

1 Histology and cell biology department Faculty of medicine Ain shams University Cairo-Egypt

2 Histology department, Faculty of Medicine, Ain Shams University

3 histology,faculty of medicine,ain shams university

Abstract

ntroduction: Ulcerative colitis is a chronic inflammatory bowel disease, that causes organ damage. There is currently no effective clinical treatment. Platelet rich plasma (PRP) and PRP- derived exosomes are a challenging therapeutic measure. measure for treatment.Aim of the Work: Evaluate the possible therapeutic role of PRP versus PRP- derived exosomes in healing of experimentally induced ulcerative colitis in rats. Material and Methods: Four groups of forty mature male albino rats were created: group I (control group); subgroup IIa (ulcerative colitis), given single transrectal injection of 2ml of 4% acetic acid, were sacrificed after 24 hours; subgroup IIb (recovery group) were left for spontaneous healing after induction of colitis then were sacrificed on day 16; group III (PRP treated),  given 0.5 ml/day of PRP intraperitoneally for 7 days, 24 hours after induction of colitis then rats were left for further 1 week, and group IV (PRP derived exosomes) given 0.5 ml/day of PRP derived exosomes intraperitoneally for 7 days, 24 hours after induction of colitis then rats were left for further 1 week. Samples from the colon were prepared for histological and immunohistochemical study.Results: Acetic acid injection significantly induced colonic damage. Loss of mucosal architecture, extensive granulation tissue containing mononuclear cellular infiltration and absence of goblet cells were detected. Treatment with PRP derived exosomes and PRP showed apparent histological improvement. A significant reduction in both area percentage of PCNA and TNF-α in these groups compared to the ulcerative colitis subgroup. However, rats treated by PRP derived exosomes showed better improvement.Conclusion: PRP derived exosomes showed better therapeutic effect than PRP in an ulcerative colitis model.

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