Histological and Biochemical study on the accelerating effect of non-cross linked hyaluronic acid and polydioxanone smooth threads on wound healing in adult albino rats.

Articles in Press

Document Type : Original Article

Authors

1 Oral and Maxillofacial Surgery Specialist, Private Practice, Cairo, Egypt

2 Assistant professor of histology and cell biology, Department of Histology and Cell Biology, Benha Faculty of Medicine, Benha University, Benha

3 Professor of histology and cell biology, Saxony Egypt University for Applied Sciences and Technology

Abstract

Background: Numerous therapeutic modalities have been developed to enhance wound healing, with ongoing advancements continuing to emerge. Among these, absorbable threads have recently garnered attention for their roles in skin rejuvenation and tissue defect repair. However, scientific validation is essential to confirm their therapeutic efficacy. Animal models provide a reliable in vivo platform for evaluating wound healing acceleration. This study aimed to assess the combined effect of polydioxanone (PDO) smooth threads and non-cross-linked hyaluronic acid (HA) injections on promoting rapid wound healing with minimal complications in adult male albino Wistar rats.

Methods and Results: Forty-five adult male albino Wistar rats were randomly assigned to five groups. Group I (negative control) comprised five rats with no incisions. The remaining forty rats underwent a standardized 1 cm full-thickness skin incision and were divided into four groups: Group II (positive control) received an intradermal saline injection prior to suturing; Group III (HA group) received 0.2 mL of non-cross-linked HA intradermally before suturing; Group IV (PDO group) underwent suturing followed by the placement of a single PDO mesothread; and Group V (HA + PDO group) received both the HA injection and PDO thread placement before suturing. After 14 days, the combined treatment group (HA + PDO) exhibited significantly enhanced healing outcomes. Histological analysis revealed improved epithelial regeneration and organized collagen deposition. Biochemical assessments corroborated these findings, indicating an enhanced capacity for wound healing and tissue repair. The synergistic application of PDO threads and HA notably accelerated the healing process without any observed adverse effects. This combination may represent a promising therapeutic strategy for future clinical wound management.

Keywords

Egyptian Journal of Histology

Articles in Press, Accepted Manuscript
Available Online from 10 May 2025

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