Hassan, R., Sayed, S., Habib, A., Yousry, M. (2025). Possible Therapeutic Effect of Chitosan Nanoparticles versus Pioglitazone Loaded Chitosan Nanoparticles on Colitis Associated with Chronic Kidney Disease in Adult Male Albino Rat Model. Histological and Biochemical Study. Egyptian Journal of Histology, 48(2), 412-431. doi: 10.21608/ejh.2024.291579.2070
Rokia Mohamad Hassan; Safinaz Salah Eldin Sayed; Alaa Serag El-dean Habib; Marwa Mohamed Yousry. "Possible Therapeutic Effect of Chitosan Nanoparticles versus Pioglitazone Loaded Chitosan Nanoparticles on Colitis Associated with Chronic Kidney Disease in Adult Male Albino Rat Model. Histological and Biochemical Study". Egyptian Journal of Histology, 48, 2, 2025, 412-431. doi: 10.21608/ejh.2024.291579.2070
Hassan, R., Sayed, S., Habib, A., Yousry, M. (2025). 'Possible Therapeutic Effect of Chitosan Nanoparticles versus Pioglitazone Loaded Chitosan Nanoparticles on Colitis Associated with Chronic Kidney Disease in Adult Male Albino Rat Model. Histological and Biochemical Study', Egyptian Journal of Histology, 48(2), pp. 412-431. doi: 10.21608/ejh.2024.291579.2070
Hassan, R., Sayed, S., Habib, A., Yousry, M. Possible Therapeutic Effect of Chitosan Nanoparticles versus Pioglitazone Loaded Chitosan Nanoparticles on Colitis Associated with Chronic Kidney Disease in Adult Male Albino Rat Model. Histological and Biochemical Study. Egyptian Journal of Histology, 2025; 48(2): 412-431. doi: 10.21608/ejh.2024.291579.2070
Possible Therapeutic Effect of Chitosan Nanoparticles versus Pioglitazone Loaded Chitosan Nanoparticles on Colitis Associated with Chronic Kidney Disease in Adult Male Albino Rat Model. Histological and Biochemical Study
1Histology and cell biology Faculty of Medicine Cairo University
2Histology Department, Faculty of Medicine, Cairo University, Cairo, Egypt
Abstract
Background: One of the pathogenic factors and a complication of chronic kidney disease (CKD) is colitis and intestinal dysbiosis. Pioglitazone hydrochloride (PIO), an antidiabetic medication which stimulates peroxisome proliferator-activated receptor-γ (PPAR-γ) & possess antioxidant and anti-inflammatory effects. Low aqueous solubility of PIO results in limited absorption and pharmacodynamics. Therefore, a drug delivery system nanoparticle is a priority. Aim of Work: Assessing the potential therapeutic impact of chitosan nanoparticles versus PIO-loaded chitosan nanoparticles on colitis associated with CKD. Materials & Methods: Twenty-nine adult male rats were categorized into 5 groups: group I, group II administered adenine daily for 3 weeks, group III treated as group II then no further intervention for 2 weeks, groups IV & V received adenine as group II then treated with chitosan nanoparticles and PIO-loaded chitosan nanoparticles respectively for 2 weeks. Biochemical analysis for stool lactobacilli, serum creatinine, colonic malondialdehyde (MDA), tumor necrosis factor-α (TNF-α) and PPAR-γ along with histological and immunohistochemical study (for claudin-1 and caspase-3) were performed. In addition to statistical analysis. Results: Group II illustrated elevated serum creatinine, colonic MDA and TNF-α as well as decreased stool lactobacilli and PPAR-γ expression. Disruption of renal architecture along with lost colonic surface epithelium, distorted intestinal crypt and inflammatory infiltration were recorded. In addition to reduced claudin-1 and increased caspase-3 immunoreactivity. As regards group III, no significant improvement was recorded in all the previously mentioned findings. While groups IV and V revealed obvious amelioration with better effects in group V. Conclusion: Both chitosan nanoparticles and pioglitazone loaded chitosan nanoparticles improved alteration of kidney function and structure and ameliorated colitis associated with CKD. While pioglitazone loaded chitosan nanoparticles illustrated superior impacts.
View on SCiNiTO
Top