Saad, B., Beheiry, E., El Sekily, N., Rostom, D., Ibrahim, S., Salem, R. (2025). The Therapeutic Potential of Platelet- Rich Plasma and/or Mesenchymal Stem Cells on Diabetic Wound Healing: Histological and Immunohistochemical Study. Egyptian Journal of Histology, 48(2), 540-572. doi: 10.21608/ejh.2024.276171.2040
Badria Hassan Saad; Eman Elazab Beheiry; Nancy Mohamed El Sekily; Dina Mohamed Rostom; Samar S Ibrahim; Rasha R. Salem. "The Therapeutic Potential of Platelet- Rich Plasma and/or Mesenchymal Stem Cells on Diabetic Wound Healing: Histological and Immunohistochemical Study". Egyptian Journal of Histology, 48, 2, 2025, 540-572. doi: 10.21608/ejh.2024.276171.2040
Saad, B., Beheiry, E., El Sekily, N., Rostom, D., Ibrahim, S., Salem, R. (2025). 'The Therapeutic Potential of Platelet- Rich Plasma and/or Mesenchymal Stem Cells on Diabetic Wound Healing: Histological and Immunohistochemical Study', Egyptian Journal of Histology, 48(2), pp. 540-572. doi: 10.21608/ejh.2024.276171.2040
Saad, B., Beheiry, E., El Sekily, N., Rostom, D., Ibrahim, S., Salem, R. The Therapeutic Potential of Platelet- Rich Plasma and/or Mesenchymal Stem Cells on Diabetic Wound Healing: Histological and Immunohistochemical Study. Egyptian Journal of Histology, 2025; 48(2): 540-572. doi: 10.21608/ejh.2024.276171.2040
The Therapeutic Potential of Platelet- Rich Plasma and/or Mesenchymal Stem Cells on Diabetic Wound Healing: Histological and Immunohistochemical Study
1Human anatomy and embryology department, Faculty of medicine, Alexandria university, Alexandria, Egypt
2anatomy and embryology department, faculty of medicine, alexandria university
3Human Anatomy and Embyrology Department, Faculty of Medicine, Alexandria University, Egypt
42Histology and cell biology department, Faculty of medicine, Alexandria University, Alexandria, Egypt
5Permanent researcher Center of Excellence for Research in Regenerative Medicine and Applications (CERRMA). Faculty of Medicine, Alexandria University.
6Anatomy department,faculty of medicine.alexandria university
Abstract
Introduction: Non-healing wounds are a significant public health issue and a significant financial drain on the healthcare system. Platelet-rich plasma (PRP) was proved to be successful in numerous experiments as it is rich in many cytokines and proteins important for wound healing. Bone marrow- derived mesenchymal stem cells (BM-MSCs) release trophic factors that control inflammation and remodelling, thus it can aid in wound healing. Objective: The aim of this study was to assess morphological, histological and immunohistochemical effects of PRP and/or BM-MSCs on cutaneous wound healing in diabetic rats. Materials and Methods: Hundred healthy male albino rats; ten rats were used as mesenchymal stem cells and PRP donors. Eighteen rats were used as negative control group (A). Seventy-two rats (B) were divided into 4 groups 18 rats each after induction of diabetes by streptozotocin (STZ) drug and formation of skin wounds; Group I: positive control group, Group II: received single dose of PRP injection in the wound, Group III: received single dose of BM-MSCs injection in the wound, Group IV: BM-MSCs plus PRP. Cutaneous wounds were inspected and photographed every 2 days of the study period for morphological study. Six rats from each group were operated upon on days 7, 14, 30 of the study for histological and immunohistochemical studies. Results: The best healing results were seen in group IV regarding morphological, histological and immunohistochemial results with a statistically significant difference between group IV and other study groups. Group III showed better healing results than in group II which was better than that of group I. Conclusion: There is a synergistic effect of combined BM-MSCs and PRP on healing of skin wounds in STZ-induced diabetic rats which is better than to use either of them alone
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