Boughdady, W., Saied, H., Radwan, S. (2025). Effect of Nano-curcumin versus Black Seed Oil on Renal Cortical Changes Induced by Aspartame in Adult Male Albino Rat. Histological and Immunohistochemical Study. Egyptian Journal of Histology, 48(1), 1-16. doi: 10.21608/ejh.2023.242282.1965
Wafaa Abd El-Azeem Abdou Boughdady; Heba Abd Elrahman Saied; Samia Hamdy Radwan. "Effect of Nano-curcumin versus Black Seed Oil on Renal Cortical Changes Induced by Aspartame in Adult Male Albino Rat. Histological and Immunohistochemical Study". Egyptian Journal of Histology, 48, 1, 2025, 1-16. doi: 10.21608/ejh.2023.242282.1965
Boughdady, W., Saied, H., Radwan, S. (2025). 'Effect of Nano-curcumin versus Black Seed Oil on Renal Cortical Changes Induced by Aspartame in Adult Male Albino Rat. Histological and Immunohistochemical Study', Egyptian Journal of Histology, 48(1), pp. 1-16. doi: 10.21608/ejh.2023.242282.1965
Boughdady, W., Saied, H., Radwan, S. Effect of Nano-curcumin versus Black Seed Oil on Renal Cortical Changes Induced by Aspartame in Adult Male Albino Rat. Histological and Immunohistochemical Study. Egyptian Journal of Histology, 2025; 48(1): 1-16. doi: 10.21608/ejh.2023.242282.1965
Effect of Nano-curcumin versus Black Seed Oil on Renal Cortical Changes Induced by Aspartame in Adult Male Albino Rat. Histological and Immunohistochemical Study
1histology department, faculty of medicine, cairo university
2Histology department, faculty of Medicine, Cairo university
3histology ,faculty of medicine ,cairo university
Abstract
Introduction: Aspartame (ASP) is a widely used an artificial sweetener. However, it is considered a risky compound causing many health problems on prolonged use. Curcumin and black seed oil (BSO) are two of the most important natural antioxidants used in alternative medicine. Recently, curcumin nano-formulations showed better bioavailability and solubility, hence delivering more therapeutic concentrations of curcumin, enhancing its efficacy. Aim of the Work: This study was carried out to investigate the effect of nano-curcumin versus BSO on aspartame-induced cortical histological changes in adult male albino rat. Materials and Methods: Forty-five rats were divided into 4 groups; 1 control and 3 experimental groups; Aspartame group: received aspartame orally (200 mg/ kg/day) for 6 weeks, nano-curcumin and BSO groups: received aspartame as the previous group concomitantly with nano-curcumin (200 mg/kg/day) and BSO (0.2 ml/kg/day) respectively orally for 6 weeks. Serum levels of urea and creatinine (Cr) and renal tissue level of superoxide dismutase (SOD) were measured. Renal sections were stained with H&E, Periodic Acid Schiff's Reaction (PAS) and immunohistochemical stains for Caspase-3, Cox-2 and Ki67. Additionally, morphometric measurements and statistical analysis were done. Results: The aspartame group showed obvious histological degenerative changes in renal corpuscles and tubules with a significant decrease in the glomerular area, mean area% of PAS and mean number of Ki-67 immuno-positive cells with significant increase in the area% of COX-2 and caspase-3 immunoreaction. Whereas, nano-curcumin and BSO groups showed apparently normal structure of the renal cortex and amelioration of the biochemical and morphometric parameters detected in the aspartame group. Conclusion: Both nano-curcumin and BSO proved a protective effect on the renal cortex against the adverse changes caused by aspartame and their efficiency was nearly similar. These findings can be considered clinically after extra human-based studies to define the suitable patient and to adjust the required dose.
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