Abdel Hameed, A., mortada, S., Morcos, M., Mahmoud, A. (2024). A Comparative Histological Study On The Therapeutic Effect of Mesenchymal Stem Cells Versus Exosomes on CCL4 Induced Liver Injury in Adult Male Albino Rats. Egyptian Journal of Histology, 47(2), 739-757. doi: 10.21608/ejh.2023.206683.1884
Asmaa Mohamed Abdel Hameed; samiha aly mortada; Mary Attia Morcos; Aya Helal Mahmoud. "A Comparative Histological Study On The Therapeutic Effect of Mesenchymal Stem Cells Versus Exosomes on CCL4 Induced Liver Injury in Adult Male Albino Rats". Egyptian Journal of Histology, 47, 2, 2024, 739-757. doi: 10.21608/ejh.2023.206683.1884
Abdel Hameed, A., mortada, S., Morcos, M., Mahmoud, A. (2024). 'A Comparative Histological Study On The Therapeutic Effect of Mesenchymal Stem Cells Versus Exosomes on CCL4 Induced Liver Injury in Adult Male Albino Rats', Egyptian Journal of Histology, 47(2), pp. 739-757. doi: 10.21608/ejh.2023.206683.1884
Abdel Hameed, A., mortada, S., Morcos, M., Mahmoud, A. A Comparative Histological Study On The Therapeutic Effect of Mesenchymal Stem Cells Versus Exosomes on CCL4 Induced Liver Injury in Adult Male Albino Rats. Egyptian Journal of Histology, 2024; 47(2): 739-757. doi: 10.21608/ejh.2023.206683.1884
A Comparative Histological Study On The Therapeutic Effect of Mesenchymal Stem Cells Versus Exosomes on CCL4 Induced Liver Injury in Adult Male Albino Rats
1Histology departement, faculty of medicine, cairo university, egypt
2faculty
3histology department faculty of medicine cairo university
4Histology faculty of medicine cairo university
Abstract
Introduction: Liver diseases are major global health burden which lead to many complications if left untreated. Aim of Work: Evaluating and comparing the therapeutic effect of bone marrow mesenchymal stem cells (BMMSCs) versus Exosomes in CCL4 induced liver injury rat model. Materials and Methods: Thirty-two adult male albino rats were divided into:Group І (Control),Group II (CCL4), Group III (Recovery),Group IV (MSCs),Group V(Exosomes). 0.1ml/kg of CCL4 in 0.1 ml olive oil was administered intraperitoneally (IP) twice weekly for six weeks for all experimental groups. MSCs were injected in the tail vein of group IV at a dose1×106 after last CCL4 injection. Exosomes were injected in the tail vein of group V at a dose 250 μg after last CCL4 injection. Animals of group II were sacrificed 6 weeks from the start of experiment. while groups(I&III&IV&V) were sacrificed 10 weeks after. ALT&AST were measured at the end of 6thand 10th week. Hematoxylin and eosin, Masson's trichrome stain,α-SMA and Caspase 3 immunostaining were performed. Mean values of AST&ALT,mean area % of collagen, mean number of α-SMA&caspase3 immunopositive cells &mean optical density of caspase 3 immunoreactivity were measured. Results: In groups II& III, hepatocytes showed cytoplasmic vacuolations with dark pyknotic nuclei.A significant increase in mean values of liver enzymes,mean area % of collagen, mean number of α-SMA & caspase 3 immunopositive cells and mean optical density of caspase 3 in group II&III compared to control group and other groups. In group IV&V hepatocytes were apparently normal, few cells showed dark pyknotic nuclei. A significant decrease in mean values of liver enzymes, mean area % of collagen, mean number ofα-SMA &caspase 3 +ve cells and mean optical density of caspase 3 in group IV&V compared to groups II&III. Conclusion: MSCs was effective as Exosomes with significant reduction of fibrosis in liver fibrosis rat model.
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