Abdel Rahman, M., Madkour, H., El-saeed El-Demerdash, F., Mahfouz, H., Ahmed Aburahma, A., Mosaad Amer, S. (2024). Histological Study on the Possible Protective Role of L-Carnitine Against Testicular Ischemia-Reperfusion Injury Induced in Rabbits. Egyptian Journal of Histology, 47(2), 694-705. doi: 10.21608/ejh.2023.199635.1873
Mohamed Abdel Rahman; Hala Madkour; Fatma El-saeed El-Demerdash; Hala Mahfouz; Abdelrahman Ahmed Aburahma; Sarah Mosaad Amer. "Histological Study on the Possible Protective Role of L-Carnitine Against Testicular Ischemia-Reperfusion Injury Induced in Rabbits". Egyptian Journal of Histology, 47, 2, 2024, 694-705. doi: 10.21608/ejh.2023.199635.1873
Abdel Rahman, M., Madkour, H., El-saeed El-Demerdash, F., Mahfouz, H., Ahmed Aburahma, A., Mosaad Amer, S. (2024). 'Histological Study on the Possible Protective Role of L-Carnitine Against Testicular Ischemia-Reperfusion Injury Induced in Rabbits', Egyptian Journal of Histology, 47(2), pp. 694-705. doi: 10.21608/ejh.2023.199635.1873
Abdel Rahman, M., Madkour, H., El-saeed El-Demerdash, F., Mahfouz, H., Ahmed Aburahma, A., Mosaad Amer, S. Histological Study on the Possible Protective Role of L-Carnitine Against Testicular Ischemia-Reperfusion Injury Induced in Rabbits. Egyptian Journal of Histology, 2024; 47(2): 694-705. doi: 10.21608/ejh.2023.199635.1873
Histological Study on the Possible Protective Role of L-Carnitine Against Testicular Ischemia-Reperfusion Injury Induced in Rabbits
1Department of Human Anatomy and Embryology, Faculty of Medicine, Sohag University, Sohag, Egypt
2Department of Pharmacology, Faculty of Medicine, Sohag University, Sohag, Egypt
3Department of Zoology and Entomology, Faculty of Science (Girl's),Al-Azhar University, Cairo, Egypt
4Department of medical biochemistry and molecular biology, Faculty of Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt
5Department of Andrology, Faculty of Medicine, Cairo University, Manial, Cairo, Egypt
6Department of Medical Histology and Cell Biology, Faculty of Medicine, Cairo University, Manial, Cairo, Egypt
Abstract
Introduction: The torsion of the testis is a surgical emergent situation which is common; however there are no effective measures to avoid damage of the testis after untwisting this torsion. L-carnitine (LC) is an essential micronutrient with strong anti-apoptotic, anti-inflammatory and antioxidant potencies that can reverse oxidative stress induced testicular damage. Aim of the Work: To evaluate the potential protective role of LC against the testicular damage induced by ischemia-reperfusion (IR) injury in rabbits. Materials and Methods: 50 adult male rabbits weighing 1300-1600 g were divided into 5 groups (10 rabbits per group). Acute IR testicular injury was induced in rabbits by twisting the left spermatic cord 720º for 2 h. Group 1: Control group and divided into two subgroups (subgroup A negative control and subgroup B positive control); Group 2: Sham-operated; Group 3: Ischemia group; Group 4: IR group (testicular IR was induced without LC); Group 5: IR+LC testicular IR was induced and LC was given by intra-peritoneal injection at a dose of 500 mg/kg immediately after torsion. At the experimental end, tissue samples of left testes were collected from all rabbits of the five groups and evaluated biochemically and histologically. Results: Ischemia-reperfusion resulted in disturbance of the oxidant/antioxidant status markers, loss of normal architecture of spermatogenic cells, apparent increase in the number of caspase-3 positive spermatogenic cells as well as abnormal fragmented chromatin material in addition to lack of halos around the sperm nuclei. However, the intra-peritoneal administration of LC immediately after torsion significantly improved these changes caused by IR on the testes. Conclusion: Administration of LC reduced the testicular damage induced by the ischemia-reperfusion.
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