The Possible Role of Platelet Rich Plasma in Treatment of the Induced Surgical Corneal Injury in Rabbits: Histomorphometric and Electron Microscopic Studies

Document Type : Original Article

Authors

1 Anatomy and Embryology, Faculty of Medicine, Ain-Shams University.Cairo,Egypt

2 Anatomy and Embryology department, Faculty Of medicine, Ain- Shams University. Cairo,Egypt

3 Anatomy and Embryology Department, Faculty of Medicine, Ain-Shams University. Cairo, Egypt

4 Anatomy Department; Faculty of medicine; ain shams university; Cairo; Egypt

5 Ophthalmology Department, Faculty of Medicine.Ain-Shams University. Cairo, Egypt

Abstract

Introduction: The health of the ocular surface that is important for visual acuity is dependent on the corneal epithelial integrity. Platelet-rich plasma is considered a therapeutic approach to corneal wounds because of its content of growth factors. These growth factors help to promote and accelerate wound healing in different tissues including the cornea
Aim of the Work: To evaluate the effect of PRP on corneal wound healing in surgically induced corneal injury in rabbits.
Material and Methods: Eighteen rabbits each weighing 2.5–3 kg were used in this experiment. Blood from the marginal vein of the ear was obtained from Rabbits assigned to be donors for PRP. The center of the cornea was marked, and the epithelium was scrabbed by an ophthalmic blade, then four incisions were made in the center of the corneal stroma. Rabbits were assigned into one of three groups: group I represented the control group that didn’t receive any treatment. Group II represented the saline group that received one drop of sodium chloride. Group III received PRP as single subconjunctival injection. Animals of all the groups were then euthanized one week after the surgery. The Cornea of all groups was extracted, processed and subjected to light, and Scanning electron microscopic examination
Results: Platelet rich plasma led to restoration of the regular arrangement of stromal collagen fibers and regular surface epithelium of the corneal wound that showed a good amount of microvilli.
Conclusion: Platelet-Rich plasma led to a more regular pattern of wound healing with better quality in induced corneal injury

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