Possible Therapeutic Effect of Gene Modified Versus Non-Modified Stem Cells on Hippocampus and Cerebral Cortex in Alzheimer’s Female Albino Rat Model Complicating Experimentally Induced Diabetes Type I

Document Type : Original Article

Authors

1 Medical histolgy departement,Faculty of medicine ,Cairo university.

2 Clinical Pathology Faculty of Medicine Cairo University

3 Medical Physiology, Faculty of Medicine, Cairo University

4 Medical Biochemistry & Molecular Biology, Faculty of Medicine, Cairo University

5 Histology and cell biology Faculty of Medicine Cairo University

6 Department of Medical Histology and Cell Biology, Cairo University, Egypt

7 Histology and cell biology, Faculty of Medicine, Cairo University

Abstract

Introduction and Objectives: The relation linking type1 diabetes (T1DM) to Alzheimer’s disease (AD) was recorded. Recently, widespread attention for gene modification of stem cells (SCs) was established. The present work aimed at investigating possible ameliorating impact of gene modified SCs on cerebral cortex and hippocampus in Alzheimer’s disease (AD) complicating T1DM.
Methods and Results: 40 female rats were classified into: In Vitro Study Group: 4 animals used to perform SCs culture and Sarco-Endoplasmic Reticulum Ca2+ATPase (SERCA) 2b preparation. Control Group (A): 6 animals. Diabetic Group (B): 10 animals received IP injection 50 mg/kg streptozotocin (STZ). Non-transfected SCs Group (C): 10 rats, received 1×106 rat SCs intravenous. SERCA2b transfected SCs Group (D): 10 rats received 1x106 transfected SCs. 52 days after proving incidence of diabetes, neurological, serum glucose, morphological, morphometric, gene quantitation, western blot and biochemical studies were performed. Neurological study expressed a decrease in group B and in group C. Group B clarified degenerated neurons in external pyramidal (EP) layer and dentate gyrus (DG). Group D revealed more obvious regression of degenerative changes than group C. An increase in area of deformed nerve cells and Congo red +ve neurons, mean area% of immunoexpression (IE) were found in groups B and C. Mean Ca ion concentration, catalase, SERCA2b gene and protein values expressed a significant (sig) decrease in groups B and C. Mean MDA and beta amyloid protein proved a sig increase in groups B and C.
Conclusions: Stem cells transfected with sarcoplasmic reticulum calcium ATPase2b gene proved pronounced therapeutic impact suggesting its potency and regenerative capacity in remarkable amelioration of Alzheimer’s disease complicating diabetes I.

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