fawzy, A., Mahmoud, R., Ibrahim, S., mahmod, A., Samy, W. (2023). Curcumin Ameliorates Obesity Associated Disorders in High Fat-Fed Male Albino Rats: Role of Hepcidin. Egyptian Journal of Histology, 46(4), 2037-2051. doi: 10.21608/ejh.2022.153618.1743
amal fawzy; Radwa Mahmoud; Safia Ibrahim; abeer mahmod; Walaa Samy. "Curcumin Ameliorates Obesity Associated Disorders in High Fat-Fed Male Albino Rats: Role of Hepcidin". Egyptian Journal of Histology, 46, 4, 2023, 2037-2051. doi: 10.21608/ejh.2022.153618.1743
fawzy, A., Mahmoud, R., Ibrahim, S., mahmod, A., Samy, W. (2023). 'Curcumin Ameliorates Obesity Associated Disorders in High Fat-Fed Male Albino Rats: Role of Hepcidin', Egyptian Journal of Histology, 46(4), pp. 2037-2051. doi: 10.21608/ejh.2022.153618.1743
fawzy, A., Mahmoud, R., Ibrahim, S., mahmod, A., Samy, W. Curcumin Ameliorates Obesity Associated Disorders in High Fat-Fed Male Albino Rats: Role of Hepcidin. Egyptian Journal of Histology, 2023; 46(4): 2037-2051. doi: 10.21608/ejh.2022.153618.1743
Curcumin Ameliorates Obesity Associated Disorders in High Fat-Fed Male Albino Rats: Role of Hepcidin
1Assistant professor of Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Egypt
2assistant prof of Physiology Department, Faculty of Medicine, Zagazig University, Egypt
3Histology departemant faculty of medecine Zagazig university
4MD of Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Egypt
Abstract
Introduction: In developed countries, obesity and iron deficiency are frequently encountered nutritional disorders. Nonalcoholic fatty liver disease (NAFLD) is a global health problem. Hepcidin is a peptide produced mainly by hepatocytes and adipocytes, which are responsible for iron metabolism, immunity, and inflammatory regulation. Curcumin, the active ingredient of turmeric. Aim of the Work: Assess the role of hepcidin in iron homeostasis and its effects on fatty liver in albino rats with HFD-induced obesity. Also explore the protective role of curcumin on hepcidin-related obesity associated disorders. Materials and Methods: Thirty adult male albino rats were divided into three groups: group I: consumed normal diet, group II: consumed high-fat diet (HFD) and group-III curcumin-treated group was fed a high-fat diet supplemented with curcumin. Body Mass Index (BMI), abdominal circumference (AC), lipid profile & some iron parameters were measured. Also, adipose-tissue-expressed hepcidin, interleukin 6 (IL-6), and tumor necrosis factor-α (TNF-α) were measured by real-time polymerase chain reaction (PCR). Hepatic tissues were stained with hematoxylin and eosin (H&E), Masson trichrome (MT) and examined microscopically. Expression levels of BAX proteins were detected by immunohistochemistry. Results: The HFD significantly stimulated adipose tissue expression of hepcidin, IL-6, and TNF-α. The HFD group had low serum iron, transferrin saturation levels and high serum ferritin. There was a significant correlation between hepcidin expression in adipose tissue and IL-6 and TNF-α expression in the HFD group. Non-alcoholic fatty liver was also identified in HFD-induced obesity group. Treatment with curcumin significantly improved the above mentioned parameters. Conclusion: Hepcidin expression in dipose tissue plays a significant role in functional iron deficiency and non-alcoholic fatty liver in obese albino rats that are improved by curcumin which has antiobesity, antioxidant, and anti-inflammatory effects.
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