Mohamed Bakr, A., Raafat, M., hammouda, G., Abdeltawab, S. (2023). The Effect of Bone Marrow Derived Mesenchymal Stem Cells Versus their Exosomes on Imiquimod-Induced Psoriasis-Like Skin Inflammation in Female Albino Rats: A Histological Study. Egyptian Journal of Histology, 46(2), 939-952. doi: 10.21608/ejh.2022.125141.1650
Ahmed Samir Mohamed Bakr; Mona Raafat; Gehad hammouda; Salwa Mohamed Abdeltawab. "The Effect of Bone Marrow Derived Mesenchymal Stem Cells Versus their Exosomes on Imiquimod-Induced Psoriasis-Like Skin Inflammation in Female Albino Rats: A Histological Study". Egyptian Journal of Histology, 46, 2, 2023, 939-952. doi: 10.21608/ejh.2022.125141.1650
Mohamed Bakr, A., Raafat, M., hammouda, G., Abdeltawab, S. (2023). 'The Effect of Bone Marrow Derived Mesenchymal Stem Cells Versus their Exosomes on Imiquimod-Induced Psoriasis-Like Skin Inflammation in Female Albino Rats: A Histological Study', Egyptian Journal of Histology, 46(2), pp. 939-952. doi: 10.21608/ejh.2022.125141.1650
Mohamed Bakr, A., Raafat, M., hammouda, G., Abdeltawab, S. The Effect of Bone Marrow Derived Mesenchymal Stem Cells Versus their Exosomes on Imiquimod-Induced Psoriasis-Like Skin Inflammation in Female Albino Rats: A Histological Study. Egyptian Journal of Histology, 2023; 46(2): 939-952. doi: 10.21608/ejh.2022.125141.1650
The Effect of Bone Marrow Derived Mesenchymal Stem Cells Versus their Exosomes on Imiquimod-Induced Psoriasis-Like Skin Inflammation in Female Albino Rats: A Histological Study
1Department of Histology and Cell Biology, Faculty of Medicine, Ain Shams University, Cairo, Egypt
2Histology and cell biology. Faculty of Medicine, Ain Shams University
3Dep. of Histology and Cell biology Ain Shams Univ.
4Department of Histology, Faculty of Medicine, Ain Shams University, Cairo, Egypt
Abstract
Introduction: Psoriasis is a chronic skin inflammatory disease. Bone marrow mesenchymal stem cells (BM-MSCs) and their derived exosomes are known for their immunomodulatory properties. Aim of the Work: To investigate the effect of locally injected BM-MSCs and their derived exosomes in female albino rats subjected to imiquimod (IMQ)-induced psoriasis-like skin inflammation. Materials and Methods: Fifty albino rats (40 females and 10 males) were used. The female animals were randomly classified into four groups; group I (control group), group II (IMQ group) where the rats received topical IMQ once daily for 5 consecutive days, group III (IMQ + BM-MSCs) where the rats received a dose of 1 million BM-MSCs on the first day only in addition to topical IMQ, and group IV (IMQ + BM-MSCs-derived exosomes) where the rats received purified concentrate of exosomes derived from BM-MSCs in addition to topical IMQ. The 10 male albino rats served as the source of BM-MSCs and their derived exosomes. After 5 days, all animals were sacrificed, and skin specimens were processed for light microscopic studies: H&E and immunohistochemical staining for Proliferating Cell Nuclear Antigen (PCNA) and Y-chromosome identification using Real-time PCR. Morphometric measurements and statistical analysis were done for the mean epidermal thickness, the mean count of PCNA-positive keratinocytes in the epidermis, and the mean area of dermal PCNA-positive reaction. Results: The general observations and microscopic examination of sections obtained from group II rats revealed psoriasis-like skin inflammatory reactions including acanthosis, parakeratosis, and marked inflammatory infiltrate. There was a statistically significant increase in the epidermal thickness and PCNA-positive reactions in group II compared to other groups. Groups III and IV showed significant improvement, however, group III showed almost normal histological structure. Conclusion: BM-MSCs and purified exosomes concentrate were shown to significantly ameliorate psoriasis-like inflammatory changes in the skin of animal models.
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