Resveratrol Antiaging Impact on Bone Marrow-Derived Mesenchymal Stem Cells in A Duration-Dependent Manner

Saad Eldien, Heba Mohamed; Mostafa, Nashwa Ahmed; Sayed, Douaa Mohamed; Mansor, Shima Gafar; Elewa, Fatma Mahmoud; Meligy, Fatma Yassine

doi:10.21608/ejh.2021.94120.1559

Resveratrol Antiaging Impact on Bone Marrow-Derived Mesenchymal Stem Cells in A Duration-Dependent Manner

Document Type : Original Article

Authors

¹ Histology and cell biology - Faculty of Medicine- Assiut University

² Assiut university

³ Clinical pathology , SECI, Assiut university , Egypt

⁴ clinical pathology department, SECI, Assiut University

⁵ Histology department Assiut university Egypt

10.21608/ejh.2021.94120.1559

Abstract

Introduction: Aging is a natural life event associated with progressive cellular and biological dysfunction. It is a cumulative process that ends with impairment of the general function of the biological system. Stem cells are unique cells with behavioral diversity making them intriguing candidates for regenerative therapy.
Resveratrol (RSV), a nonflavonoid polyphenol phytoalexin with a stilbene structure, was first extracted from the root of white hellebore, and it was widely used in traditional Chinese medicine.
Aim of Work: We tried to postulate if resveratrol had therapeutic effects on aged MSCs and whether these effects were duration dependent or not
Materials and Methods: We achieved our study on bone marrow-derived mesenchymal stem cells (BM-MSCs) derived from 15-month aged rats. We used 40 rats; subdivided into four equal subgroups; (A) control, (B) received resveratrol for one week, (C) received resveratrol for two weeks, and (D) received resveratrol for one month. Morphological changes were tracked, surface marker expression was measured, and various parameters of proliferation kinetics were used; colony-forming unit (CFU), population doubling time (PDT) "3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT)", "DNA index" and budding uninhibited by benzimidazole-related 1. (BubR1) immunocytochemical staining were implemented.
Results: We reached significant results in improving morphological changes, proliferation kinetic parameters, immunophenotypic flow cytometric CD90 marker expression, DNA index, and anti BubR1antibody immunocytochemical staining even after two weeks of treatment.
Conclusions: Resveratrol had flourishing effects of BM-MSCs on aging cells. It promoted growth, proliferation, improved viability, established genetic stability, and improved MSC aging parameters documented in marked duration-dependent patterns.

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