Ali, S., Elmarakby, D., Amer, S., Abdallah, H. (2023). Histological Study on the Effect of Insulin and Platelet-Rich Plasma on Skin Wounds Induced in Diabetic Adult Male Albino Rats. Egyptian Journal of Histology, 46(1), 263-279. doi: 10.21608/ejh.2021.86084.1527
Salwa Metwaly Ali; Dalia Elmarakby; Sarah Mosaad Amer; Hanan Abdallah. "Histological Study on the Effect of Insulin and Platelet-Rich Plasma on Skin Wounds Induced in Diabetic Adult Male Albino Rats". Egyptian Journal of Histology, 46, 1, 2023, 263-279. doi: 10.21608/ejh.2021.86084.1527
Ali, S., Elmarakby, D., Amer, S., Abdallah, H. (2023). 'Histological Study on the Effect of Insulin and Platelet-Rich Plasma on Skin Wounds Induced in Diabetic Adult Male Albino Rats', Egyptian Journal of Histology, 46(1), pp. 263-279. doi: 10.21608/ejh.2021.86084.1527
Ali, S., Elmarakby, D., Amer, S., Abdallah, H. Histological Study on the Effect of Insulin and Platelet-Rich Plasma on Skin Wounds Induced in Diabetic Adult Male Albino Rats. Egyptian Journal of Histology, 2023; 46(1): 263-279. doi: 10.21608/ejh.2021.86084.1527
Histological Study on the Effect of Insulin and Platelet-Rich Plasma on Skin Wounds Induced in Diabetic Adult Male Albino Rats
1Department of Histology, Faculty of Medicine, Cairo University, Cairo, Egypt
2Histology department, Faculty of Medicine, Cairo University, Cairo, Egypt
3Histology department,faculty of medicine,Cairo university
Abstract
Abstract Background: Diabetes mellitus (DM) is a metabolic disorder that has many complications like cardiovascular diseases, diabetic nephropathy and unhealed diabetic skin wounds. Aim of the work: To evaluate the possible curative effect of insulin and platelet rich plasma (PRP) on diabetic skin wounds induced in adult male albino rats. Materials and Methods: 50 adult male albino rats, with an average body weight 150-200 gm, were divided into (control and experimental groups). The control groups (20 rats) that were equally subdivided into two groups (control I: normal healthy rats with intact skin and control II: diabetic rats with non-treated skin wounds managed with local intradermal saline injections 500 μL once daily at wounds margins). Experimental groups (30 rats) that were equally further subdivided into three groups; Insulin treated group I: diabetic rats with local intradermal insulin injections 0.5 U/500 μL once daily at wounds margins. PRP treated group II: diabetic rats with local intradermal PRP injections 500 µl as a single dose at wounds margins. Combined treated group III: diabetic rats with local intradermal combined PRP and insulin injections with 2 hours intervals. Each group in the control and experimental groups was further subdivided into 2 subgroups A and B according to the time of scarification after 14 and 21 days post wounds induction respectively. Results: The experimental groups showed significant improvement in wound closure, epidermis regeneration and increased collagen deposition as compared to non -treated control group II. The insulin and combined treated groups showed increased collagen deposition as compared to PRP treated group, however, the combined group showed the best results. Conclusion: Local combined treatment with insulin and PRP showed a rapid rate of diabetic wounds closure.
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