Effect of Morin on Fluoxetine (Prozac)-Induced Structural Changes of Mice Skeletal Muscle: Light and Electron Microscopic Study

Kandeel, Samah; Kandeel, Mahmoud; S. Estfanous, Remon

doi:10.21608/ejh.2021.92787.1549

Effect of Morin on Fluoxetine (Prozac)-Induced Structural Changes of Mice Skeletal Muscle: Light and Electron Microscopic Study

Document Type : Original Article

Authors

¹ Histology Department, Faculty of Medicine, Tanta University

² Department of Pharmacology, Faculty of Veterinary Medicine, Kafrelshikh University, Kafrelshikh 33516, Egypt

³ Anatomy and Embryology department, Faculty of Medicine, Tanta University, Egypt

10.21608/ejh.2021.92787.1549

Abstract

is natural polyphenol with different health benefits due to its antioxidant, and anti-inflammatory actions.
Aim of the Work:This work designed to evaluate the effect of morin on fluoxetine (Prozac)-induced structural changes of mice skeletal muscle by means of light and electron microscopic study.
Materials and Methods: 30 mice (50-70 gm) were used for 3 weeks and divided into 6 groups (5 rats each); Group 1: Control, Group 2: Morin low dose (MLD): mice received low dose of morin 30 mg/kg/day orally dissolved in distilled water, Group 3: Morin high dose (MHD): mice received high dose of morin 100 mg/kg/day orally dissolved in distilled water, Group 4: Prozac group (PR): mice received an intraperitoneal injection of 0.06 mg PR/mice/day in normal sterile saline. Group 5: Prozac +Morin low dose: (PR+MLD): mice that received an intraperitoneal injection of 0.06 mg PR/mice/day in normal sterile saline preceded by one hour with a low dose of morin (30 mg/kg/day) dissolved in distilled water orally, and Group 6: Prozac +Morin high dose: (PR+MHD): mice that received an intraperitoneal injection of 0.06 mg PR/mice/day in normal sterile saline preceded by one hour with a high dose of morin (100 mg/kg/day) dissolved in distilled water orally. Oral doses were by an intragastric tube.
Results: PR group showed vacuolations, cellular infiltrations, small, dark central nuclei at H&E sections, decreased PAS +ve reaction, significantly increased collagen area percentage, significantly increased Cytochrome-C area percentage, myofibrils with wide separation, accumulations of mitochondria of different sizes and shapes, and degenerated nucleolar membrane with vacuolated nucleoplasm at electron microscope. On the other hand, these previous changes were ameliorated by morin dose dependently.
Conclusion: Morin dose dependently could protect against fluoxetine induced skeletal muscle degenerative changes in mice.

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