Saad, S., Isaac, M., Kamar, S. (2022). A Comparative Histological Study to the Effect of Adipose Derived Mesenchymal Stem Cells Versus Virgin Coconut Oil in Skin Wound Healing of Adult Male Albino Rat. Egyptian Journal of Histology, 45(1), 162-172. doi: 10.21608/ejh.2021.56439.1411
Shereen Saad; Mary Refaat Isaac; Sherif A Kamar. "A Comparative Histological Study to the Effect of Adipose Derived Mesenchymal Stem Cells Versus Virgin Coconut Oil in Skin Wound Healing of Adult Male Albino Rat". Egyptian Journal of Histology, 45, 1, 2022, 162-172. doi: 10.21608/ejh.2021.56439.1411
Saad, S., Isaac, M., Kamar, S. (2022). 'A Comparative Histological Study to the Effect of Adipose Derived Mesenchymal Stem Cells Versus Virgin Coconut Oil in Skin Wound Healing of Adult Male Albino Rat', Egyptian Journal of Histology, 45(1), pp. 162-172. doi: 10.21608/ejh.2021.56439.1411
Saad, S., Isaac, M., Kamar, S. A Comparative Histological Study to the Effect of Adipose Derived Mesenchymal Stem Cells Versus Virgin Coconut Oil in Skin Wound Healing of Adult Male Albino Rat. Egyptian Journal of Histology, 2022; 45(1): 162-172. doi: 10.21608/ejh.2021.56439.1411
A Comparative Histological Study to the Effect of Adipose Derived Mesenchymal Stem Cells Versus Virgin Coconut Oil in Skin Wound Healing of Adult Male Albino Rat
1Anatomy department, Faculty of Medicine , Ain Shams University
2Anatomy Department, Faculty of Medicine, Ain Shams University.
3Abbaseya
Abstract
Background: Skin wounds comprise a clinical load for many patients. Owing to their availability and ability for differentiation, adipose-derived stem cells (ADSCs) are considered a promising line in the tissue regenerative medicine. Virgin coconut oil (VCO) has been also reported to help in wound healing by decreasing time of complete re-epithelization. However, there was lack in literature about histological details. Aim: The study aimed to compare the effect of ADSCs versus natural VCO in wound healing. Material and Methods: Thirty adult male rats were used in three experimental groups, ten rats in each. All animals were exposed to excisional skin wound injury. Group I was used as wounded untreated group. Group II was injected intra-dermally with 1x106 ADSCs in four injection sites around the wound, while group III was treated with topical application of 0.5 gm VCO ointment. After 2 weeks, all rats were sacrificed. Macroscopic shrinkage of wounds was recorded and compared. Skin samples were harvested and processed for light microscopic examination using H. & E. & Masson ̍s trichrome stains. Morphometric measurements were done for both epidermal thickness & collagen fibers area percentage. Data was analyzed statistically. Results: Histological examination of group II revealed regenerated epidermis and dermal skin appendages. Masson ̍ s trichrome stained sections revealed thick collagen bundles running in different directions. In Group III, epidermis was thin with few developing dermal skin appendages. Masson ̍ s trichrome stained sections showed dense parallel bundles of collagen fibers. Conclusion: Virgin coconut oil enhanced skin wound healing. However, ADSCs proved to be more efficient in regaining mature skin structure.
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