Ismail, D., ShamsEldeen, A., Rashed, L., Shama, A., Ashour, S., Aboulkhair, A. (2021). Cardioprotective Potential of Zinc and Vitamin E Against Isoprenaline-Induced Myocardial Infarction in Albino Rats by Targeting Autophagy: A Histological and Biochemical Study. Egyptian Journal of Histology, 44(2), 450-464. doi: 10.21608/ejh.2020.36645.1331
Dalia Ibrahim Ismail; Asmaa Mohammed ShamsEldeen; Laila Ahmed Rashed; Ashraf Aly El Desoky Shama; Sara Salama Ashour; Alshaymaa Gamal Aboulkhair. "Cardioprotective Potential of Zinc and Vitamin E Against Isoprenaline-Induced Myocardial Infarction in Albino Rats by Targeting Autophagy: A Histological and Biochemical Study". Egyptian Journal of Histology, 44, 2, 2021, 450-464. doi: 10.21608/ejh.2020.36645.1331
Ismail, D., ShamsEldeen, A., Rashed, L., Shama, A., Ashour, S., Aboulkhair, A. (2021). 'Cardioprotective Potential of Zinc and Vitamin E Against Isoprenaline-Induced Myocardial Infarction in Albino Rats by Targeting Autophagy: A Histological and Biochemical Study', Egyptian Journal of Histology, 44(2), pp. 450-464. doi: 10.21608/ejh.2020.36645.1331
Ismail, D., ShamsEldeen, A., Rashed, L., Shama, A., Ashour, S., Aboulkhair, A. Cardioprotective Potential of Zinc and Vitamin E Against Isoprenaline-Induced Myocardial Infarction in Albino Rats by Targeting Autophagy: A Histological and Biochemical Study. Egyptian Journal of Histology, 2021; 44(2): 450-464. doi: 10.21608/ejh.2020.36645.1331
Cardioprotective Potential of Zinc and Vitamin E Against Isoprenaline-Induced Myocardial Infarction in Albino Rats by Targeting Autophagy: A Histological and Biochemical Study
1Histology Department, Faculty of Medicine, Cairo University, Cairo, Egypt
2Physiology Department Faculty of medicine Cairo University
3Department of Biochemistry and Molecular Biology ,Faculty of Medicine, Cairo University,Ciro, Egypt
4Department of Surgery, Anesthesiology and Radiology. Faculty of veterinary medicine Cairo University
5Department of Biochemistry and molecular biology. Faculty of medicine Cairo University
6Histology Department, Faculty of Medicine, Cairo University
Abstract
Background: Myocardial infarction (MI) is a leading cause of morbidity and mortality. It is associated with oxidative stress, apoptosis and inflammation. Zinc (Zn) and vitamin E (VE) are known to exert antioxidant and anti-inflammatory effects. Aim of the Work: Evaluate the cardioprotective potential of Zn, VE and their combination against isoprenaline (ISO)-induced myocardial infarction in adult male albino rats. Materials and Methods: Forty rats divided into five groups; I (control), II (ISO group): rats were injected subcutaneously (SC) with ISO (100 mg/kg) on the 20th and 21st days at interval of 24 h. Groups III (Zn group), IV (VE group) and V (ZE group): rats received respectively daily Zn (30 mg/kg), VE (100 mg/kg) or combination of both orally for 21 days and injected with ISO as group II. On the 22nd day, electrocardiography, biochemical and histological studies were done. Myocardial sections were subjected to H&E, caspase-3 and beclin 1 immunohistochemical stains. This was followed by morphometric and statistical analysis. Results: Group II exhibited significant electrocardiographic and biochemical changes compared to the control; deterioration of cardiac function with elevated cardiac enzymes, MDA, TNF-α and mTOR, in addition to reduced SOD, IL-10 and AMPK. Myocardial sections showed disturbed architecture with marked inflammatory infiltration and significantly increased caspase-3 and decreased beclin 1 immunoexpression. Groups III and IV revealed decreased cardiac enzymes, MDA, TNF-α and mTOR, in addition to elevated SOD, IL-10 and AMPK. Myocardial sections showed nearly normal histology with significantly decreased caspase-3 and increased beclin 1 immunoexpression. Group V presented the most protection with results significant to both groups III and IV. Conclusion: Combined Zn and VE pretreatment proved to have protective effect against ISO-induced MI more than using either of them alone regarding the electrocardiography, biochemical and histological parameters and this was through targeting autophagy and modulating its AMPK-mTOR pathway