Kandeel, S., Balaha, M., S. Estfanous, R. (2021). The Possible Ameliorative Effect of Granulocyte Colony Stimulating Factor on Rotenone -Induced Changes in the Retinal Photoreceptors of Rats: Histological and Immunohistochemical Study. Egyptian Journal of Histology, 44(2), 309-321. doi: 10.21608/ejh.2020.32962.1316
Samah Kandeel; Mohamed Balaha; Remon S. Estfanous. "The Possible Ameliorative Effect of Granulocyte Colony Stimulating Factor on Rotenone -Induced Changes in the Retinal Photoreceptors of Rats: Histological and Immunohistochemical Study". Egyptian Journal of Histology, 44, 2, 2021, 309-321. doi: 10.21608/ejh.2020.32962.1316
Kandeel, S., Balaha, M., S. Estfanous, R. (2021). 'The Possible Ameliorative Effect of Granulocyte Colony Stimulating Factor on Rotenone -Induced Changes in the Retinal Photoreceptors of Rats: Histological and Immunohistochemical Study', Egyptian Journal of Histology, 44(2), pp. 309-321. doi: 10.21608/ejh.2020.32962.1316
Kandeel, S., Balaha, M., S. Estfanous, R. The Possible Ameliorative Effect of Granulocyte Colony Stimulating Factor on Rotenone -Induced Changes in the Retinal Photoreceptors of Rats: Histological and Immunohistochemical Study. Egyptian Journal of Histology, 2021; 44(2): 309-321. doi: 10.21608/ejh.2020.32962.1316
The Possible Ameliorative Effect of Granulocyte Colony Stimulating Factor on Rotenone -Induced Changes in the Retinal Photoreceptors of Rats: Histological and Immunohistochemical Study
1Histology Department, Faculty of Medicine, Tanta University
2Pharmacology Department, Faculty of Medicine, Tanta University, Egypt
3Anatomy and Embryology department, Faculty of Medicine, Tanta University, Egypt
Abstract
Introduction: Rotenone is an odorless isoflavone used as an insecticide, and piscicide. It is a potent inhibitor of the mitochondrial respiratory chain complex I leading to oxidative stress that ends with cell death. Granulocyte colony-stimulating factor (G-CSF) is a glycoprotein cytokine and a hematopoietic growth factor, produced by many cells. It promotes cell proliferation, survival, and mobilization. It also has anti-inflammatory and anti-apoptotic actions. Aim: This study aimed to investigate the ameliorative effect of G-CSF in a rat model of rotenone-induced retinal photoreceptors’ changes. Materials and Methods: 25 male Wistar rats were used and were divided in to 3 groups; Control group; ROT group: by which rats received 30 mg/kg/day rotenone suspended in 0.25 ml 0.5% CMC orally beginning from 1st day of the experiment till 28th day; and G-CSF group: which was the rotenone group rats that further treated with 50 ìg/kg/day G-CSF in 0.1 ml 5% dextrose subcutaneous for 15 days beginning from the 29th day till the 43rd day of the experiment. Results: H & E results of the ROT group showed outer & inner segments with extensive structural damage, nuclear fragmentation, besides karyorrhexis, and karyolysis, together with vacuolations of the synaptic region. Also, significant increase of caspase-3 while, decrease of N-cadherin immunohistochemical expression. The EM findings revealed extensive degenerative changes in the all segments of the photoreceptors besides significantly increased malondialdehyde levels. Conclusion: G-CSF can ameliorates rotenone-induced degenerative structural changes in rat’s retinal photoreceptors. This was through its anti-apoptotic and anti-inflammatory actions. So, G-CSF is a promising neuroprotective drug for retinal diseases and/or injuries.