Synergistic protective effect of dexamethasone and erythropoietin on experimentally induced cochlear toxicity in albino rats [Histological and immunohistochemical study].

Articles in Press

Document Type : Original Article

Authors

1 department of histology and cell biology, faculty of medicine, Benha university, Egypt faculty of medicine, Benha National university, Egypt

2 Department of Histology and Cell Biology, Faculty of Medicine, Benha University, Egypt. faculty of medicine, Benha national university, Egypt

3 histology, faculty of medicine, benha university faculty of medicine, Benha national university, Egypt

Abstract

Background: Cisplatin (CIS) is averse -neoplastic substitute pro the therapy of solid tumors. Conversely, an extraordinary incidence of harsh ototoxicity is prevalant in patients remedied by CIS. Dexamethasone (DX) is a synthetic steroid equivalent expended for the treatment of distinctive inner ear diseases. Erythropoietin (ER) had well-established erythropoietic function plus strong anti-inflammatory, anti-apoptotic validity.
Purpose: The principle of this study was to assess the otoprotective effects of dexamethasone (DX) and erythropoietin (ER) administration in the CIS- aggravated ototoxicity in rats.
Methods: Thirty-five rats apportioned into: Control group, CIS group, CIS + DX group received an intraperitoneal injection of dexamethasone before CIS for 3 consecutive days, CIS + ER group, received an intraperitoneal injection of ER before CIS for 3 days and CIS + DX + ER group, cisplatin obtained and pretreated with dexamethasone and erythropoietin. The cochleae were subjected to the histological and immunohistochemical methods.
Results: The DX group exhibited a moderate protective effect against CIS- provoked cellular ototoxicity and remarkably reduced the histopathological finding. Pretreatment with ER effectually preserved inner and outer hair cells and supporting cells in the ѻrgan of Cѻrti as compared tѻ the effects distinguished with CIS. Quantification of spiral ganglion neurons by immunohistological anti-caspase 3 significantly shown that ER-treated rats had a significantly less spiral ganglion neuron harm than CIS rats. The synergistic dose of DX and ER have better picture nearly near normal histological structure.
Conclusion: These results demonstrated that each DX and ER revolutionize the histological represent of cochlea simply together were effective and better in protection and treatment of cisplatin- provoked ototoxicity.

Keywords

Egyptian Journal of Histology

Articles in Press, Accepted Manuscript
Available Online from 10 June 2025

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