Document Type : Original Article
Authors
1
Professor of Anatomy and embryology ,faculty of medicine Cairo university
2
Professor of Anatomy and Embryology Faculty of Medicine, Cairo University.
3
Lecturer of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University
4
Lecturer of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt
5
lecturer of Anatomy and embryology faculty of medicine Cairo university, Giza, Egypt
6
Assistant Lecturer of Anatomy and Embryology, Faculty of Medicine, Cairo University, Cairo, Egypt.
7
Lecturer of Anatomy and Embryology, Kasr Alainy ,Cairo University
Abstract
Zinc oxide nanoparticles (ZnO NPs) are widely consumed orally in different applications, food additives, drug and gene delivery systems within the field of medicine. However, the safety of these nanoparticles largely depends on factors such as the duration of exposure, the dose, or the concentration to which an individual is subjected. The lung tissue is liable to damage due to environmental and non-environmental toxic substances. Consequently, this research aimed to check out the potential toxic effects of orally administered ZnO NPs on a critical organ, namely the lungs, while also exploring the possible protective or therapeutic effect of resveratrol in mitigating these harmful effects. Fifty albino rats were divided into five groups: control, sham, toxicity, protective resveratrol and therapeutic resveratrol. LM examination, immune-histochemical; Bcl2, Bax, caspase-3 and TNF-alpha, histo-morphometric assessment, MDA, SOD, GPx and NO were examined. Western blotting for IL1 beta and IL4 proteins in addition to gene expression of HO-1 were also evaluated. Administration of resveratrol; whether protective or therapeutic; significantly reduced lung parenchymatous destruction, interstitial collagen fibers, Bax, Caspase 3, TNF-alpha expression levels, MDA and NO tissue levels. Whereas area percentage of Bcl2, SOD and GPx tissue levels were increased significantly compared to that of ZnO NPs toxicity groups. Protective resveratrol displayed better results than therapeutic one in ameliorating lung toxicity. These findings implied that resveratrol alleviates the histolopathological and biochemical effects of ZnO NPs-induced lung toxicity, furthermore, it enhanced the pro-apoptotic and antioxidant parameters which are triggered in toxicity groups. It is recommended to study the prophylactic role of resveratrol and the maximum safe dose and duration of use regarding oral ZnO NPs.
Keywords
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