THE IMPACT OF ROSEMARY "ROSMARINUS OFFICINALIS L." NANOFORMULATION ON THE GROWTH AND SURVIVAL OF HUMAN DENTAL PULP STEM CELLS (IN VITRO STUDY)

Articles in Press

Document Type : Original Article

Authors

1 Department of Basic Dental Science, National Research Centre, Cairo, Egypt. Stem Cell Laboratory, Center of Excellence for Advanced Sciences, National Research Centre, Cairo, Egypt.

2 Associate professor of oral biology- faculty of dentistry- Cairo university

3 Department of Basic Dental Science, National Research Centre, Cairo, Egypt.

4 Oral biology department faculty of dentistry Cairo university. Egypt

Abstract

Background: During normal physiological cell function reactive oxygen species (ROS) production occur. When ROS exceeds the normal level, it will inhibit cell proliferation and other cellular function. That’s why, antioxidants supplementation in the culture media are very important. The ongoing study’s aim is to examine the role of rosemary nano formulation as a potential antioxidant on human dental pulp stem cells (hDPSCs) culturing and proliferation. Methods: In this experimental study, rosemary nanoparticles were fabricated by ultra-sonication technique. DPSCs were isolated from sound completely impacted 3rd molars and were treated by rosemary nanoparticles. The survival rate and proliferation of cells were evaluated using trypan blue staining, MTT assay and apoptosis assessment by Annexin V staining. Stemness of cells was evaluated by PCR assays for stemness markers (OCT3/4, SOX2 and NANOG). ROS detection kit (OxiSelect In Vitro ROS/RNS Assay Kit) was used to assess the antioxidant potential of rosemary nanoparticles on DPSCs. Eventually, the effect of rosemary nanoparticles on DPSCs’ migratory potential was evaluated using in-vitro cell migration assay. Results: Rosemary nanoparticles supplementation in the culture media significantly upregulated the proliferation rate and survival of DPSCs and also significant upregulation in the expression of (OCT3/4, SOX2 and NANOG). Further, rosemary nanoparticles significantly reduced ROS production and significantly enhanced DPSCs migration potential. Conclusion: our results indicated that rosemary nanoparticles could be considered an efficient promoter of DPSCs’ proliferation and migration with a potent antioxidant activity that significantly exhibit ROS scavenging potential, making them a suitable candidate for stem cell culture and future regenerative applications while combating oxidative stresses.

Keywords

Egyptian Journal of Histology

Articles in Press, Accepted Manuscript
Available Online from 12 March 2025

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