Histological study on the effect of different concentrations of adipose derived stem cells in enhancing wound healing of excisional skin wounds in adult albino rats

Articles in Press

Document Type : Original Article

Authors

1 Department of histology, kasr el ainy medical school, Cairo University

2 Dep. of Histology and cell biology Cairo University

3 Histology Department, Faculty of Medicine, Cairo University, Cairo, Egypt

4 histology department, kasr al ainy medical school, cairo university

Abstract

Background:One medical condition that is hurting patients is impaired wound healing. The goal of wound healing is to repair damaged tissue in response to an injury.
Aim of work:The current study's objective was to assess and contrast the regenerative and healing capacities of high and low concentrations of adipose-derived stem cells (ADSCs) on adult albino rats' excisional wound healing.
Materials and Methods: 48 mature male albino rats weighing an average of 180–200 grams (6-7 weeks old) were enrolled in this investigation. They were split up into 4 groups, with 12 rats in each: Control group: rats without any wounds make up control group I. Group II: injured rats with open wounds on their skin. Group III: injured rats given low-dose ADSC therapy (LC-ADSCs).Group IV:injured rats given high-concentration ADSC therapy (HC-ADSCs). Using the scarification time as a guide, each group was equally divided into two subgroups, a and b, after seven and fourteen days, respectively. Using a surgical scalpel, a full-thickness square wound that reached the deep fascia was made and removed. Cotton pads and a dressing were applied to the wound to further protect it.
Results: PKH26-labeled cells were noted in the ADSCs-treated groups. Groups that were treated showed significant improvement regarding wound closure, epidermal regeneration, area percentage of collagen deposition as well as CD31 to detect the endothelial cells of dermal vessels immune reactivity when compared with the wounded untreated group. The rats that received HC-ADSCs illustrated better results in wound contraction, collagen deposition, dermal and epidermal regeneration, CD31 immune positive reaction and early termination of inflammatory reaction when compared with untreated wounded group and the group received LC-ADSCs
Conclusion: Both concentrations of ADSCs improved the healing of wound in comparison with the untreated wounded group. However, HC-ADSCs had better outcome and was more effective in healing of wound

Keywords

Egyptian Journal of Histology

Articles in Press, Accepted Manuscript
Available Online from 25 September 2024

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