2024-03-29T11:51:58Z
https://ejh.journals.ekb.eg/?_action=export&rf=summon&issue=7369
Egyptian Journal of Histology
1110-0559
1110-0559
2019
42
2
Histological Study on the Effect of Hyaluronic Acid Injection (Dermal Filler) on the Skin of the Adult and Aged Albino Rat
Marian
Aziz
Elham
Gamal El Din
Soheir
Ahmed
Mohamed
Ahmed Mekway
Background: Injectable dermal fillers became more popular for improving skin contour defects such as wrinkles, lines, and depressed acne scars. Aim of the work: This work aimed to study different skin changes induced by dermal filler injection in adult and aged albino rat. Material and Methods: Forty five female albino rats were used. The animals were divided into three groups: Group I (Control): Included twenty adult rats. Group ІІ: Included fifteen adult rats, they received an injection of 0.1 ml of hyaluronic acid (HA). The animals were subdivided into three subgroups according to time of sacrification , five rats each: Subgroup IIa: after 7 days. Subgroup IIb: after 60 days. Subgroup IIc: after 90 days. Group ІІІ: Included ten aged rats, they were subdivided into two subgroups, five rats each: Subgroup IIIa: (Control aged group). Subgroup IIIb: the rats were given an injection of HA and were sacrificed after 30 days.Results:Thin skin of HA injected animals revealed significant (< 0.05) increase in the thickness of subcutaneous tissue (SC) and significant increase in collagen and elastic fibers, and significant increase in the CD34 positive endothelial cells as compared to control and aged rats. Active fibroblasts at the site of injection showing euchromatic nuclei. Their cytoplasm contained well developed rER, numerous mitochondria, many vesicles, and centrioles.Conclusion: Hyaluronic acid filler injection stimulated vascularization and enhanced fibroblasts to synthesize collgen and elastic fibers in SC. This protected the skin from aging features in adults,and play a role in improvement of skin defects in aged rats.
CD34
dermal filler
Hyaluronic acid
Rats
senile skin
2019
06
01
245
261
https://ejh.journals.ekb.eg/article_47553_1965a631242474d627f916e0206c7293.pdf
Egyptian Journal of Histology
1110-0559
1110-0559
2019
42
2
Comparative Histological Study on the Protective Effect of Folinic Acid versus Fish Oil on the Growing Bone of Methotrexate-Treated Young Rats
Hend
Bassiouny
Noha Mohammed
Afifi Amin
Ehab
Ibrahim
Rokia
Hassan
Caspase-3
fish oil
folinic acid
growth plate
Methotrexate
2019
06
01
262
275
https://ejh.journals.ekb.eg/article_47559_35987d0922bc703f9c05288e8c4e1ec4.pdf
Egyptian Journal of Histology
1110-0559
1110-0559
2019
42
2
Autologous-Versus Allogeneic-Bone Marrow Cell Grafting in Prevention of Obstructive Nephropathy in Rats
Islam
Abdel Fattah
Manal
AL Badawi
Mona
Mohamed
Background: The increasing number of patients suffering from chronic renal disease (CRD) and end-stage renal disease (ESRD) are a challenge for the development of ingenious therapies. Bone marrow (BM) stem cells are an attractive therapy in the treatment of CRD. Aim of the work: This study aims to compare autologous and allogeneic BM cells in treatment of renal damage in a model of unilateral ureteral obstruction (UUO). Materials and methods: Sixty adult female Sprague-Dawley rats were divided randomly into four groups of 15 animals each: Rats of first group served as (Sham group). The sham-operated animals underwent identical surgical procedures, except that the left ureter was manipulated without ligation and sectioning. Rats underwent UUO with direct intra-renal injection of saline were considered as (UUO) group. The remaining rats were injected with autologous (Auto group) or allogeneic (Allo group) BM cells directly into the renal parenchyma at the same day of operation. In Allo group, the ages of the donor rats were approximately the same as the recipient rats. Results: In the present study, we showed that both types BM cells provided a protective effect and ameliorated interstitial fibrosis as reflected by reduced tubular dilation, decreased interstitial expression of .-smooth muscle actin and significant decline in expression of both transforming growth factor-.1 and caspase-3 in the tubular cells. Autologous BM cells reduced the tubular dilation index from 36.6 (as in UUO rats) to 8.13. Allogeneic BM cells reduced tubular dilation index to 16.87. Conclusion: Our results show that a single intra-renal administration of autologous or allogeneic BM cells strongly associated with a reno-protective role with enhanced proliferation and reduced apoptosis of tubular cells promoted by UUO.
2019
06
01
276
284
https://ejh.journals.ekb.eg/article_47562_25efb60cc71c8f1c1f30d55c88a20685.pdf
Egyptian Journal of Histology
1110-0559
1110-0559
2019
42
2
The Possible Protective Role of Methionine against Sodium Fluoride-Induced Pancreatic Changes in the Adult Male Albino Rat: A Histological, Immunohistochemical and Morphometric Study
Dorreia
Zaghloul
Wail
Gad-El-Rab
Reneah
Bushra
Ali
Farahat
Background: Excess fluorides intake produces histopathological changes of many organs. Methionine is a potential natural antioxidant against oxidative radicals. Aim of the Work: To evaluate the possible protective role of methionine against sodium fluoride (NaF)-induced pancreatic toxicity. Material and Methods: Thirty 3-months (200-250gm) adult male albino rats were divided into three equal groups: group I (control), group II (Fluoride group) and group III (Fluoride+methionine group). Control group; was given 1ml distilled water. Fluoride group; was given 10 mg NaF/kg b.w. Fluoride+methionine group; was given 10 mg NaF/kg b.w. and 2 mg methionine/rat. All the treatment was given orally by gastric tube once daily for 35 days. After anesthesia, all groups were sacrificed. The pancreatic specimens were prepared for light and electron microscopic studies and anti-insulin antibody immunohistochemical staining. The mean numbers of zymogen granules and insulin positive β-cells of all groups were counted.Results: The mean numbers of zymogen granules and insulin positive β-cells of the fluoride group were significantly decreased when compared to control. The pancreatic specimens of the fluoride group revealed congested blood vessels, extravasated blood cells, vacuolated pancreatic acini, loss of the acinar cell architecture, dilated rough endoplasmic reticulum and degenerated mitochondria. By anti-insulin antibodies immunohistochemistry, there was a weak positive reactivity in the fluoride treated group when compared to control. The concomitant administration of NaF and methionine improved these changes. Conclusion: The concurrent administration of NaF and methionine ameliorates the structural alterations developed in the pancreas following excess NaF intake.
albino rat
methionine
pancreas
Sodium fluoride
2019
06
01
285
296
https://ejh.journals.ekb.eg/article_47522_caf80b1f7d4e2305fc83f7e44c5dca15.pdf
Egyptian Journal of Histology
1110-0559
1110-0559
2019
42
2
Effect of tartrazine on gastric mucosa and the possible role of recovery with or without riboflavin in adult male albino rat
Walaa
Elwan
Marwa
Ibrahim
Background: Tartrazine is one of the azo dyes that are the most common artificial food colors widely used in many food products. Tartrazine is used in many developing countries without strict regulations.Aim of the study: to investigate the effect of tartrazine on the gastric mucosa and to evaluate the possible role of recovery after its withdrawal with or without riboflavin in rat.Materials and Methods: Twenty-four adult male albino rats were equally divided into 4 groups; Control, riboflavin, tartrazine-treated group (orally administered 200 mg/kg/day tartrazine for 60 days), tartrazine-recovery group (orally administered 200 mg/kg/day tartrazine for 60 days then left without treatment for another 60 days) and tartrazine-recovery and riboflavin group (that were orally administered tartrazine for 60 days then stopped and followed with riboflavin for another 60 days). Glandular stomach specimens were processed for histological and immunohistochemical techniques.Results: Tartrazine-treated group depicted variable degrees of mucosal lesions with significant decrease in its thickness. Parietal cells with vacuolated cytoplasm and irregular nuclei, and vacuolated chief cells with pyknotic nuclei were detected. Dilated congested blood vessels and aggregated mononuclear cells were observed. Ultrastructural examination showed parietal and chief cells with condensed nuclei and dilated rough endoplasmic reticulum. Tartrazine-recovery group showed almost intact gastric mucosa. Tartrazine-recovery with riboflavin group showed a near normal gastric mucosa. Both Ki67 and iNOS-immunohistochemical expression showed a statistically significant increase upon tartrazine administration coupling to a significant decrease in Periodic-Acid-Schiff expression. Tartrazine-recovery group still revealed significant differences in these parameters compared to the control, while tartrazine-recovery with riboflavin showed non-significant differences from the control.Conclusion: Tartrazine affected the stomach and was alleviated by stopping it. Combined recovery along with riboflavin was more efficient on the recovery of gastric mucosa.
iNOS, Ki67
Riboflavin
Stomach
Tartrazine
2019
06
01
297
311
https://ejh.journals.ekb.eg/article_47564_d693527911b5d58c32580156d354b119.pdf
Egyptian Journal of Histology
1110-0559
1110-0559
2019
42
2
A Comparative Histological and Immunohistochemical Study of the Effect of Oxandrolone and Whey Protein on Disuse Induced Muscle Atrophy in old aged male rats
Samiha
Mortada
Rahma
Abou Elnour
Amal
Fares
Dina
Hisham
Background: Periods of immobilization are often associated with pathologies and/or aging. These periods of disuse muscle atrophy could worsen the pathology or elderly frailty. Exercise, the best way to recover muscle mass, is not always applicable. Aim of Work: This study aimed to compare the therapeutic effect of oxandrolone versus whey protein on disuse induced muscle atrophy in old aged male rats with low testosterone level and mobility limitation.Materials and Methods: Twenty four aged male albino rats were included: 6 rats as control (group I). Eighteen rats were subjected to hind limb suspension (HLS) for 2 weeks then were divided into: 6 rats as group II (disuse atrophy) and 12 rats as group III (treated group) which was equally subdivided into: group IIIa (oxandrolone treated) and IIIb (whey protein treated) where oxandrolone 1.23 mg/kg and whey protein 9.25 gm/kg were given orally daily for 4 weeks. Rats were sacrificed after 6 weeks. Gastrocnemius muscle weight and total protein content were measured. Muscle specimens were stained with Hematoxylin and Eosin, PAS and immunohistochemical staining for Myogenin. Morphometric measurements of type I and type II fibers number and cross sectional area (CSA), number of myogenin positive cells (differentiated satellite cells) were taken and statistically analyzed. Results: Group II showed atrophic angulated muscle fibers and significant increase in type II fibers compared to the control. Subgroup IIIa showed muscle fibers with large diameter. Many rounded vesicular nuclei were observed among muscle fibers. A significant increase in type I fibers and number of myogenin positive cells as compared to the control and subgroup IIIb. Conclusion: Oxandrolone and whey protein treated subgroups showed improved muscle mass, CSA, total protein content, histological architecture of muscle fibers and increase of satellite cells number. However, oxandrolone showed better therapeutic potential in ameliorating disuse muscle atrophy.
Muscle atrophy
myogenin
HLS
oxandrolone
whey protein
2019
06
01
312
323
https://ejh.journals.ekb.eg/article_47566_bf911638ab6ba4885c69616701de7375.pdf
Egyptian Journal of Histology
1110-0559
1110-0559
2019
42
2
Ameliorative Effect of Omega-3 on Energy Drinks - Induced Pancreatic Toxicity in Adult Male Albino Rats
Asmaa
El Desouky
Ahmed
Abo Zaid
Ghada
El Saify
Dalia
Noya
Background: The world wide increasing popularity of the energy drinks and the lack of information about their possible hazardous effects on health is a matter of controversy and research. The aim of this study is to assess the histological and histochemical effects of energy drinks on the pancreas of adult male albino rats and the possible protective effect of omega-3. Material and Methods: Fifty adult male albino rats were randomly divided into 4 groups. First group is control. Second group (Omega-3 treated) rats received omega-3 at a dose of 300 mg /kg/day orally for 4 weeks. Third group (Red Bull treated) rats received Red Bull at a dose of 10 mg/kg/day orally for 4 weeks then they were randomly subdivided into two equal subgroups: IIIA, rats were sacrificed after 24 h of the last dose and in IIIB (Recovery group), rats were sacrificed after 4 weeks of the last dose .Fourth group (Omega-3 and Red Bull treated group) rats received Red Bull at a dose of 10mg/kg/day and Omega-3 at a dose of 300mg/kg/day for 4 weeks. At sacrifice, blood samples were drawn for biochemical study and pancreas specimens were prepared for histological and histochemical study. Results: Energy drink had no significant effect on the animal weight (P = 0.055), but there was highly significant increase in the pancreatic weight (P = 0.001) and in mean blood glucose level (P = 0.000). There were signs of β cells overstimulation. Histological and histochemical study of the pancreatic sections revealed multiple deleterious effects of the energy drink on the acinar and the islet cells. These changes were reversible as shown in the recovery group. Co-administration of Omega-3 showed marked protection of the pancreatic acini and the islets of Langerhans. Conclusion: Omega-3 administration has a highly protective effect on the pancreatic tissue against the hazardous effects of the energy drinks.
Energy drink
Insulin Resistance
islets of langerhans
omega-3
pancreas
2019
06
01
324
334
https://ejh.journals.ekb.eg/article_47568_c51c598eb1fc92cfe1e408b5a44a09fd.pdf
Egyptian Journal of Histology
1110-0559
1110-0559
2019
42
2
Effect of Obesity on The Lung of Adult Male Albino Rat with Evaluation of The Possible Protective Role of Pomegranate : Light and Electron Microscope Study
Essam
laag
Sadika
Tawfik
Basem
El-Shafey
Background: Pomegranate is rich in bioactive compounds, mainly polyphenols and anthocyanins, with known health benefits, due to its high antioxidant capacity. Aim of the work: Is to study the protective effects of pomegranate on the histologiacl changes induced by obesity on the lung of the adult male albino rat. Materials and methods: The present study was carried on 40 adult male albino rats divided into 4 equal groups: control group kept on standard diet for 3 months, group II (kept on standard diet and pomegranate juice in a dose 20ml/Kg orally for 3 months), group III (received high fat diet for 3months), and group IV (received both high fat diet and pomegranate juice at the same previous doses and period). At the appropriate time, the specimens were taken and prepared for light and electron microscopic study. Results: Light microscope examination revealed structural alterations in group III in the form of inflammatory cellular infiltration with desquamated epithelial cells inside the lumens of bronchioles and alveoli. Collapsed alveoli with fragmented elastic fibers in their wall and intra-alveolar cellular infilteration were detected. Congested blood vessels with increased lipid droplets inside pneumocyte type II were also noticed. Increased peri-bronchiolar, peri-vascular and inter-alveolar collagen deposition with interrupted elastic lamina in bronchioles and around alveoli were also detected. Ultrastructural examination of spicemens taken from group III showed increased lipid droplets compressing the nuclei with disorganized multilamellar bodies in type II pneumocytes. Neutrophils and eosinophils were seen inside the alveoli. Amelioration of the previous histological changes was detected in group IV after weight reduction.Conclusion: it was concluded that pomegranate has anti-obesity effects which ameliorated the previous histological changes induced in the lung by receiving high fat diet.
lung
Obesity
pomegranate
2019
06
01
335
345
https://ejh.journals.ekb.eg/article_47533_6925cd2eca4fe51401df889feb2d3ec8.pdf
Egyptian Journal of Histology
1110-0559
1110-0559
2019
42
2
Effects of Anabolic Steroids on the Histological Structure of Renal Cortex of Adult Male Albino Rats and the Possible Protective Role of Taurine
Mary
Isaac
Introduction: Misuse of anabolic androgenic steroids (AAS) has become a widespread health problem with subsequent multisystem adverse effects. However, among their adverse effects, renal toxicity remains one of the least discussed.Aim of work: To study the histopathological effects of Nandrolone Decanoate (ND) abuse on renal cortex and examine the possible protective role of taurine. Materials and methods: Thirty-six adult male albino rats, aging 4-6 months and weighing 200 - 250 gms, were used in this study. Animals were divided equally into three groups; Group I (control group): subdivided into Subgroup IA: negative control and Subgroup IB: rats received intramuscular 0.5ml sesame oil twice weekly for six weeks and Subgroup IC: received 100 mg/kg/day Taurine by gavage, for six weeks. Group II (ND Group): received intramuscular 5 mg/kg ND, dissolved in sesame oil, twice weekly for six weeks. Group III (Taurine and ND Group): given both Taurine and ND in the same dose and duration as subgroup IC and group II respectively. At the end of the experiment, venous blood samples were collected from the tail vein for biochemical assays. Kidneys were dissected out and processed for light and electron microscopic examination. Results: Examination of group II revealed marked congestion, obliteration of Bowman’s space and lumina of both proximal and distal tubules. Ultrastructural distortion was evident in glomerular filtration barrier and tubular lining cells. Statistically significant biochemical changes were also observed. However, group III showed almost preserved glomerular and tubular architecture with few persistent changes. Biochemical results also revealed marked improvement. Conclusion: Marked renal cortical affection with renal functional deterioration were noted following ND. Taurine exerted marked reno-protection thus, it would be also of great help to protect AAS users against possible renal hazards in case their AAS use was inevitable.
Anabolic steroids
renal cortex
taurine
2019
06
01
346
357
https://ejh.journals.ekb.eg/article_47570_c929fd34a632b7816847441b2ff466f1.pdf
Egyptian Journal of Histology
1110-0559
1110-0559
2019
42
2
Effect of Black and Green Tea on Experimental Sodium Oxalate-Induced Nephrotoxicity in Adult Male Mice
Hussein
Ibrahim
Abd El Ghany
Moustafa
Hassan
Elgebaly
Background: Oxalate is a frequent component in foods from plant origin. Extraordinary concentrations of oxalate in urine leads to several renal pathological disorders. Green tea and black tea have several beneficial effects attributed to their polyphenols which act as metal chelators and scavengers of free radicals. Aim: The present study aimed to clarify the possible ameliorating impact of black and green tea on renal tissue damage caused by oxalate. Material and Methods: Thirty male mice were divided into six groups: Group I (control) ,Group II were injected with sodium oxalate, group IIIwere fed with black tea extract and group IV were fed with black tea extract and injected with sodium oxalate, group V were fed with green tea extract and group VI were fed with green tea extract and injected with sodium oxalate. The black or green tea extracts were given orally for 10 days (2ml/100g/day ). Sodium oxalate (100 mg/kg) were injected as a single intraperitoneal dose at the11th day and animals sacrificed 12 h later. At the end of experiment, blood samples and kidney specimenswere obtained from all groups. Serum urea and creatinine analysis were done. The renal tissue sections were stained with H&E and PAS stains and examined. Immunohistochemical investigation for iNOS and NF-κB and morphometric analysis were also implemented. Results:Microscopically, the examined kidneys in oxalate group revealed degenerated and necrotic tubular cells ,tubular dilatation, protein casts and translucent oxalate crystals and interrupted tubular brush border and basal laminae. Furthermore, serum urea and creatinine were elevated and iNOS and NF-κB immunoreaction were highly positive. The aforementioned changes were reversed in group IVand VI.Conclusion: Black and green tea extracts could protect against oxalate-induced nephrotoxicity due to their antioxidant characters.
Black tea
green tea
Immunohistochemistry
Nephrotoxicity
oxalate
2019
06
01
358
368
https://ejh.journals.ekb.eg/article_47521_e7a891fa9438964d29f09017d60b3b18.pdf
Egyptian Journal of Histology
1110-0559
1110-0559
2019
42
2
Histological Study of the Effect of Platelet Rich Plasma on Experimentally Induced Skeletal Muscles Injury in Adult Male Albino Rats
Shimaa
Badr
Reda
Elbakary
Essam
Laag
Naglaa
Sarhan
Nafisa
Elbakary
Background: Muscle injuries are common and may be associated with impaired functional capacity, especially among athletes. Ordinary lines of treatment result in improper healing of the damaged tissue with prolonged time for recovery, so it was essential to try new lines of treatment for these injuries to get advantageous effect on the healing process. One of these new treatments is platelet-rich plasma (PRP), which is used to improve healing of many lesions such as skin and sports injury. Aim of the work: This work was carried out to evaluate the role of platelet rich plasma in healing of experimentally induced skeletal muscle injury in adult male albino rat. Materials and methods: Thirty adult male albino rats were divided Into: Group I: donor group to obtain PRP from their peripheral blood and muscle specimens from their gasterocnemius muscle. Group II: was exposed to bilateral gastrocnemius muscles injury and was left without treatment. Group III: Bilateral gastrocnemius muscles were injured and immediately treated locally with PRP intramuscularly. Muscle specimens were excised after 1, 7and 21 days from the onset of injury and were processed for light and electron microscopic examination. Results: PRP treated group demonstrated early formation of many myotubes starting from the 7th day after injury onwards in association with ultrastructural features of activated satellite cells together with restoration of normal histological features of muscle fibers. Conclusion: local injection of PRP into the injured gastrocnemius muscle resulted in early activation, proliferation and differentiation of satellite cells resulting in earlier and enhanced myogenesis.
Muscle injury
platelet rich plasma
satellite cells
2019
06
01
369
380
https://ejh.journals.ekb.eg/article_47519_5bcf4d607d833c971ba5d18bd8aecd37.pdf
Egyptian Journal of Histology
1110-0559
1110-0559
2019
42
2
The Toxic Effect Of Sildenafil Citrate On Adult Albino Rat Testis And The Possible Protective Role Of Royal Jelly (Histological And Immunohistochemical Study)
Hanan
Elkerdasy
Ali
Mohamed
Background: Phosphodiesterase inhibitor was appearing as a magic tool in treatment of erectile dysfunction, however, it might lead to harmful effects in high dose and long term administration. The aim of the work: This study aimed to evaluate the toxic effect of sildenafil citrate on the histological structure of the testis of adult albino rats and the possible protective role of royal jelly. Materials and methods: Thirty five adult male albino rats were divided into 3 groups; the control group divided into (subgroup Ca, Cb and Cc), experimental group divided into (subgroup Ea and Eb) and treated group divided into (subgroup Ta and Tb). In the experimental group sildenafil citrate was given at a dose of 9 mg/kg in subgroup Ea and 13.5mg/kg in subgroup Eb orally daily for 30 days. In the treated group rats were treated by royal jelly at a dose of 100 mg/kg for 30 days orally after stoppage of sildenafil citrate. In the present study, the sperm count, histological and immunohistochemical studies were evaluated. Result : Daily oral administration of sildenafil citrate (9mg/kg and13.5 mg/kg ) for 30 day had reduced the sperm count and induced apparent degeneration with detached, distorted germ cell and congested blood vessels with significant decrease in Proliferating cell nuclear antigen Labeling Index (PCNA- LI). After royal jelly administration, there was an improvement in sperm count and histopathological changes induced by sildenafil citrate with a significant increase in PCNA -LI. Conclusion: Long-term daily use of Sildenafil citrate produced histological changes in the testis and reduced the spermatogenesis with a significant decrease in sperm count and these side effects can be prevented by using royal jelly.
PCNA-LI
royal jelly
Sildenafil citrate
testes
2019
06
01
381
392
https://ejh.journals.ekb.eg/article_47518_b0ebb240d25e6652c8c4bf235a8a103a.pdf
Egyptian Journal of Histology
1110-0559
1110-0559
2019
42
2
The possible protective effect of Gardenia Jasminoides extracts on the dentate gyrus changes in an Alzheimer-induced model in adult male albino rats: Histological and Immunohistochemical study
Maha
Gazia
Introduction: Alzheimer's disease (AD) is the most common diagnosed form of senile dementias, a neurodegenerative disease resulting in cognitive impairment in the elderly. Geniposide, extracted of Gardenia Jasminoides, is widely used herb for brain diseases and reduce amyloid plaques deposition which is an intricate process in AD.Aim of the work: demonstrate the histological changes in the dentate gyrus of hippocampus in an Alzheimer-induced model and the possible neuroprotective effect of Gardenia Jasminoides extract.Materials and methods: Thirty six adult male albino rats(4-6 months) , weighing180-200g divided into 3 groups. Group I the control group, group II given aluminum chloride dissolved in distilled water, (300mg/kg/day) orally for one month. Group (III) given Geniposide in distilled water,at a dose of (100mg/kg/day) orally concomitant with aluminum chloride at the same dose and period of the previous group. Cognitive functions were assessed by the passive avoidance test. Temporal lobes processed for H&E , congo red stains. Immunohistochemical study for detection of (GFAP) and (INOS). Tau protein can be phosphorylated by glycogen synthase Kinase-3β(GSK-3 β). Western blot assay to investigate the total protein expression glycogen synthase Kinase-3β(GSK-3 β) . Comet assay to detect DNA damage.Results: Alzheimer-induced model group showed significantly impaired cognitive performance which was improved after treatment with Geniposide extract. Changes in the dentate gyrus apparent increase in immature neurons, disturbed granular cell layer, distortion of pyramidal cells that appeared with pyknotic nuclei. Positive immunoreaction for GFAP in astrocytes were also observed. The ultra structural study exhibited neurons with apoptotic features, heterochromatic nuclei and their cytoplasm contained dilated cisternae of rough endoplasmic reticulum. Gardenia extracts ameliorated histological alterations.Conclusion: Gardenia Jasminoides extracts significantly ameliorated the neuro- degenerative alterations induced in the Alzheimer model. Daily supplements of Gardenia Jasminoides extracts might be of benefit for the elderly people at risk of developing AD.
Gardenia
Jasminoides
Alzheimer
Aluminum
chloride
2019
06
01
393
407
https://ejh.journals.ekb.eg/article_26115_80153a0dd35d3b49ef0c9dc5034bb029.pdf
Egyptian Journal of Histology
1110-0559
1110-0559
2019
42
2
A Comparative Study between the Effect of Nerve Growth Factor and All-Trans Retinoic Acid versus their combined use on Taxol Induced Peripheral Neuropathy in Adult Male Albino Rat
amany
Abdelrahman
nesreen
Abd Elhaliem
hassan
elnady
asma
lotfy
hoda
Moghazy
Introduction: Peripheral neuropathy is a major side effect of chemotherapy. Nerve growth factor induces peripheral nerve regeneration. Retinoids play a significant role in neural growth. This study aimed to investigate the curative effect of either recombinant human nerve growth factor (rh-NGF) or all trans-retinoic acid (ATRA) alone or together on taxol induced peripheral neuropathy. Materials and methods: Fifty two male albino rats were divided into five groups. Group 1was control group. Group 2 was i.p. injected with taxol (2mg/kg) on days 1, 3, 5, and 7. Group 3 received taxol as in group II then at the 10th day it was i.p. injected with NGF (10ug/kg daily for 20 days). Group 4 received taxol as in group II then at the 10th day it was i.p. injected with ATRA (25mgkg daily for 20 days). Group 5 received taxol as in group II then it was i.p. injected with rh-NGF and ATRA at the 10th day with the same previous dose. Neurophysiological assessment was done for sensory and motor nerve conduction velocity and amplitude as well as thermal pain threshold. Sections of sciatic nerve were prepared for immunohistochemichal and electron microscopic studies. Results: Taxol injected animals exhibited signs of peripheral neuropathy at the 10th day after the first dose of taxol. There was a significant decrease in sensory and motor conduction as well as prolongation in thermal pain threshold. Myelinopathy (splitting, ovoid fragments and invagination) and axonapathy (compressed irregular axoplasm, myelin figures and destructed mitochondria) were seen in the histological examination. Glial fibrillary acidic protein immunoreactive positive cells were significantly increased. rh-NGF and ATRA could ameliorate the electrophysiological and the histological changes. Their combined use had the best result. Conclusion: This study concluded that the use of rh-NGF and ATRA combination could improve taxol induced peripheral neuropathy in male albino rats.
NGF
ATRA
taxol
GFAP
peripheral neuropathy
2019
06
01
408
424
https://ejh.journals.ekb.eg/article_25922_c2d0b0f3d41e6c0e4f965175f28028a5.pdf
Egyptian Journal of Histology
1110-0559
1110-0559
2019
42
2
The potential protective role of flaxseed extract on ventral prostate in a rat model of streptozotocin-induced diabetes: A histological and immunohistochemical study
Amira
Kassab
Background: Diabetes mellitus is a chronic disorder that can affect the reproductive system leading to fertility dysfunction. The prostate plays an important role in the reproduction and it is a frequent target for diabetic complications. Flaxseed is a promising alternative in many chronic diseases due to its health benefits. It has a proposed role in controlling diabetes due to its wide pharmacological activities. im: Evaluation of the possible protective role of flaxseed extract on the ventral prostate gland of diabetic rats. Materials & Methods: Fifty adult male albino rats were used as a control group and experimental group that received a single intra-peritoneal injection of streptozotocin (60 mg/kg). The experimental rats with confirmed diabetes mellitus were subdivided into: diabetic group that kept without treatment and diabetic–flaxseed group (400 mg/kg/day orally). After four weeks, specimens of the ventral prostate lobes were processed for histological and immunohistochemical study using antibodies against Bcl-2, α-smooth muscle actin and androgen receptor. Results: In comparison with the control, specimens of the diabetic group showed glandular epithelial atrophy with thickening of the fibromuscular stroma. Reduced epithelial height and a statistically significant decrease in Bcl2 immunoexpression were observed in the epithelial cells. A statistically significant increase in the collagen fibers and α-SMA immunoexpression was seen in the stroma. Androgen receptor immunoreactivity was significantly decreased in both epithelial and stromal cells. In contrast, minimal changes appeared in diabetic-flaxseed group that received flaxseed extract. Conclusion: Diabetes induced structural alterations in the ventral prostate gland of rats. Administration of flaxseed extract attenuated these effects and partially preserved the glandular structure.
Diabetes mellitus
Flaxseed
prostate gland
2019
06
01
425
436
https://ejh.journals.ekb.eg/article_26174_01c405c0429a2ff595113fd000bf1041.pdf
Egyptian Journal of Histology
1110-0559
1110-0559
2019
42
2
The Effect of Diet Coke and Monosodium Glutamate on the Cerebellar Cortex of Adult Male Albino Rats. Histological and Immuno-histochemical Study
Noha
Abd El-Wahed
Esmat
Geith
Nagwa
Kalleny
Hadwa
Abd Al-Khalek
Background: The consumption of fast- food is increasing among children, adolescents and adults. Carbonated drinks as Diet Coke are widely consumed with fast food. One of the thousands of chemicals used in our new high-tech foods is the monosodium glutamate (MSG). Aim of the study: This study was conducted to investigate the effect of Diet Coke and monosodium glutamate salt either separately or in combination on the cerebellar cortex of adult male albino rats.Materials and Methods: Forty adult male albino rats were divided into four groups. Group I: control group. Group II (Diet Coke group) which was subdivided into: subgroup IIA: each rat received 2.5 ml of Diet Coke twice daily by oral gavage and subgroup IIB: rats received Diet Coke instead of water throughout the day. Group III (MSG group): rats received MSG salt solution (3 gm MSG / kgm) by oral gavage once daily. Group IV (combination group) rats received combination of MSG solution and Diet Coke. This group was subdivided into two subgroups. Subgroup IVA: rats received MSG salt solution in the same dose as group III and 2.5 ml of Diet Coke twice daily, by oral gavage. Subgroup IVB: rats received MSG salt solution in the same dose as group III and Diet Coke instead of water throughout the day. At the end of the experiment (21 days) cerebella were dissected out and processed for histological, immunohistochemical and morphometric studies. Results: Histological examination of the cerebellar cortex revealed that both Diet Coke and MSG either separately or in combination resulted in degenerative changes that were more significant in the combination group.Conclusion: Diet Coke and MSG induced degenerative changes in the cerebellar cortex that was more significant in their combination.
Cerebellum
diet coke
MSG
2019
06
01
437
452
https://ejh.journals.ekb.eg/article_47579_4b35e8312d47bc9c5a8441c46dee4ed1.pdf
Egyptian Journal of Histology
1110-0559
1110-0559
2019
42
2
Histological effect of high versus low dose isotretinoin and possible protective role of Tiron on skin of adult male albino rat
Maha
Soliman
Hala
El-Haroun
Maisa
Kafafy
doaa
mohamed
Background: Isotretinoin is one of the most effective drugs in acne treatment. It is retinoic acid derivative. Systemic administration of isotretinoin may induce many adverse effects. Tiron has antioxidant properties and could ameliorate skin damage and cell apoptosis. Aim of the work: To evaluate the adverse effect of high versus low dose isotretinoin on the skin of adult albino rats and the probable protective effect of tiron. Material and Method: Sixty adult male albino rats were divided into five groups: control group I (DMSO and saline), tiron treated group II (300 mg/kg orally), isotretinoin treated group III (6 mg/kg /day IP), isotretinoin treated group IV (3 mg/kg /day IP), and protective group V which treated with isotretinoin and tiron. Rats of the last group were subdivided into 2 equal subgroups (V a & V b) which received isotretinoin at a dose of 6mg and 3mg /kg/day respectively with tiron. At the end of the studied period, rats were then sacrificed and the samples were taken for histological, histochemical, immunohistochemical and morphometric studies.Results: Animals treated with isotretinoin at a dose of 6mg/kg/day showed thinning of all layers of epidermis. Epidermal cells were degenerated, exfoliated with marked reduction in cellular proliferation and associated with ill-defined basement membrane. Sebaceous glands showed a marked reduction in surface area and some cells appeared degenerated and dermis revealed loss of its normal architecture and increased densely packed collagen fibers seen by Mallory trichrome stain. These degenerative changes were less apparent in administration of isotretinoin at a lower dose of 3mg/kg/day.Concomitant administration of tiron and isotretinoin induced an observable improvement protection against these changes compared to control, especially in low dose. Conclusion: The pronounced skin damage induced by isotretinoin can be ameliorated by joined therapy of tiron and isotretinoin.
Isotretinoin
skin
tiron
2019
06
01
453
466
https://ejh.journals.ekb.eg/article_47580_911aaa489fcf6cba0903570d2cc99eaa.pdf
Egyptian Journal of Histology
1110-0559
1110-0559
2019
42
2
A Comparative Study of the Ameliorative Effect of Doxorubicin with Vitamin E versus Liposomal Doxorubicin on the Left Ventricular Histological and Immunohisochemical Changes Induced by Doxorubicin in Adult Male Albino Rats
fadia
abdo
Fayza
Ahmed
Mohamed
Shaheen
Samar
Mostafa
Background and Aim: The use of Doxorubicin (Dox) to treat various tumors is limited by its cardiotoxicity. This study aimed to compare the cardioprotective potential of vitamin E versus liposomal-Dox against cardiotoxic effects on the structure of left ventricle. Materials and Methods: Fifty male albino Wistar rats (180-220g) were divided into control (I), Dox (II), vitamin E with Dox (III) and liposomal-Dox (IV) groups. Groups II, III and IV received Dox and liposomal-Dox (3mg/kg) at days 1, 3, 5, 7, 9 and 11. Group III received Vitamin E (100mg/kg) daily with Dox administration. At day 12, blood was collected and left ventricles were dissected and prepared for LM and EM study. Results: Serum lactate dehydrogenase, creatine phosphokinase and creatine kinase-MB increased by 2.5 folds in Dox-treated rats compared to control rats, but decreased in vitamin E and liposomal-Dox groups compared to Dox group. The maximal decrease was in liposomal-Dox group with values near to control. LM examination of left ventricle from Dox-group showed hemorrhagic areas between widely separated cardiomyocytes containing pyknotic nuclei. Inflammatory cells and adipocytes were seen in the interstitium. EM of Dox group showed variable sized mitochondria with ruptured cristae inbetween fragmented myofibrils. LM and EM examination of ventricles from vitamin E and liposomal-Dox groups showed mild changes with liposomal-Dox group appeared near to control. Left ventricular fiber diameter was decreased in Dox group compared to control group with nearly normal diameter in liposomal-Dox group. Area percent of collagen fibers increased and the optical density of desmin immune-expression was reduced in Dox group compared to control and liposomal-Dox groups which were alike. Conclusion: Dox-induced structural changes of rat ventricle were diminished by concomitant vitamin E intake. Liposomal-Dox administration-only- was more effective in reducing these changes; hence, it can accomplish a successful clinical target to decrease Dox-associated cardiotoxicity
cardiotoxicity
Doxorubicin
histology
liposomal-Dox
Vitamin E
2019
06
01
467
481
https://ejh.journals.ekb.eg/article_26695_3928799ec05ff1819c58791d4914b2f3.pdf
Egyptian Journal of Histology
1110-0559
1110-0559
2019
42
2
A histological and immunohistochemical study of the effect of platelet- rich plasma on a corneal alkali burn in adult male albino rat
Heba
Sharaf eldin
Marwa
Ibrahim
Noha
Elswaidy
Background: Ocular chemical injuries are an emergency that needs immediate and intensive evaluation and treatment. Platelet- rich plasma (PRP) has become a common treatment in the field of ophthalmological surgery.Aim of the study: to evaluate and compare the alleviating effect of a single dose of PRP injection applied either after 2 hours or 48 hours on an alkali-induced corneal burn.Materials & Methods: Thirty adult male albino rats were equally divided into 5 groups; Control, PRP group, alkali-burn group, alkali-burn+ PRP-treated after 2hours group and alkali-burn+ PRP-treated after 48hours group. Corneal specimens were processed for different histological and immunohistochemical techniques. Results: Alkali burnt cornea revealed focal discontinuity and denudation of corneal epithelium alternating with focal disorganization and stratification. Bowman’s membrane appeared irregular with focal disruption. The Descemet’s membrane appeared thinned out and disrupted. Mononuclear cellular infiltration and invasion with small blood vessels were observed. Upon intervention with PRP after 2hours of alkali-burn, a near normal corneal histological structure was observed. Yet upon intervention with PRP after 48hours of alkali-burn, a disturbed corneal histological structure with vacuolated epithelium and some nuclear changes were detected. A highly significant difference in the immunohistochemical staining for detection of P53, Ki67, MMP-1 and MMP-9 was detected upon alkali burn compared to the control, whereas a non-significant difference was observed in group IV compared to the control group, yet a significant difference was detected between group V and the control group. Conclusion: A single dose PRP injection had an effective alleviating effect on alkali-induced corneal burn, yet the PRP injection after 2hours of the burn was more efficient in restoring the corneal healthy surface rather than its application after 48 hours.
Alkali-burn
cornea
Immunohistochemistry
PRP
2019
06
01
482
495
https://ejh.journals.ekb.eg/article_26802_81e5fd683dc5f131bd5110f72628ebd9.pdf
Egyptian Journal of Histology
1110-0559
1110-0559
2019
42
2
Histological Study on the Effect of Adipose Mesenchymal Stem Cells Derived Microvesicles and the Role of its RNA Content on Experimentally-Induced Ulcerative Colitis in Albino Rats
Manal
Abdel Mohsen
Marwa
Ahmed
Background: Ulcerative colitis (UC) is a bowel inflammatory illness, which shows remission and deterioration episodes of abdominal pain and bloody diarrhea. Nowadays, treatment options are not satisfactory other than their various complications. Adipose tissue derived mesenchymal stem cell microvesicles (MSC-MVs) are considered an emerging promising alternative therapeutic agent. Aim of the work: Evaluation of the therapeutic potential of MSC-MVs on acetic acid (AA) induced UC in addition to the potential role of its RNA content. Materials and Methods: Forty adult male albino rats were equally divided to 4 groups: group I (control), group II (colitis), group III (MVs treated) and group IV (RNase-MVs treated). Colitis was induced by intracolonic AA enema for groups II, III and IV. On day three after enema a single intravenous injection with either PBS, MSC-MVs or RNase treated MSC-MVs was administered to groups II, III and IV, respectively. Seven days later, colon specimens were harvested and cut equally into two parts. The proximal parts were used for biochemical study to measure myeloperoxidase enzyme (MPO) levels. The distal parts were processed for H&E, Alcian blue, iNOS and COX-2 immunohistochemistry. Morphometric and statistical analysis were done. Results: Group III showed improved histological features of UC and marked increase in the mean area percent and optical density of mucin. Also, it showed significant decrease in iNOS and COX-2 immunoreaction and MPO levels. Conclusion: MSC-MVs administration improved UC features. RNA content of the vesicles proved to have a crucial role in such protective effects. Consequently, MSC-MVs could be a promising tool in UC treatment.
Cox2
iNOS
mesenchymal stem cell microvesicles
rNase
ulcerative colitis
2019
06
01
496
512
https://ejh.journals.ekb.eg/article_27111_0ca99bc4df354d8f5d9a486338d3cdaa.pdf
Egyptian Journal of Histology
1110-0559
1110-0559
2019
42
2
Amelioration of Titanium Dioxide nanoparticles induced injury on the cerebellum of the adult mice by a hydromethanolic root extract of Withania Somnifera
2019
06
01
512
513
https://ejh.journals.ekb.eg/article_47584_e9b133e4df575f24a8919a452c73874d.pdf