@article { author = {Ismail, Dalia and Yousry, Marwa}, title = {The effectiveness of resveratrol in protection against histological alterations induced by hyperprolactinemia in reproductive organs of female albino rats}, journal = {Egyptian Journal of Histology}, volume = {41}, number = {2}, pages = {123-139}, year = {2018}, publisher = {Egyptian Society of Histology and Cytology in Cooperation with The Egyptian Knowledge Bank (EKB).}, issn = {1110-0559}, eissn = {2090-2417}, doi = {10.21608/EJH.2018.13834}, abstract = {Introduction: Hyperprolactinemia (HPRL) commonly causes primary amenorrhea and reproductive disorders. Resveratrol (RES), a natural phenol, possesses antioxidant, anti-inflammatory and anti-apoptotic effects. However, its protective role on the histological structure of female reproductive organs in case of HPRL remains unclear.Aim of the work: This study aimed at evaluating the protective effect of RES against the HPRL induced by metoclopramide in female albino rats.Materials and Methods: Female rats that showed three regular estrus cycles (determined by vaginal smears) were divided into three groups. Group I was the control group. Group II rats were given oral metoclopramide (2 mg/Kg/day) for 28 consecutive days. Group III rats were given oral RES (20 mg/kg/day) for 28 days concurrently with metoclopramide. Serum superoxide anion (O2−), prolactin (PRL), estradiol (E2), progesterone, follicle stimulating hormone (FSH) and luteinizing hormone (LH) were assessed, in addition to measurement of tissues tumor necrosis factor-alpha (TNF-α). Sections from uteri, oviducts and ovaries were stained with hematoxylin and eosin and caspase-3 immunohistochemical stain and were subjected to morphometric and statistical studies.Results: Group II showed significant increase in O2−, TNF-α and PRL, with significant decrease in the sex hormones. There were histological alterations in the uteri, oviducts and ovaries, with significant increased caspase-3 immunoexpression compared to the control. While, in group III, there was significant decrease in O2−, TNF-α and PRL, with significant increase in the sex hormones. The uteri, oviducts and ovaries showed minimal changes, with significant reduction in caspase-3 immunoreactivity compared to group II.Conclusion: Administration of resveratrol ameliorates the oxidative stress, inflammation, hormonal assay alterations, histological changes and apoptosis resulted from induced HPRL in female rats.}, keywords = {apoptosis,female reproductive organs,hyperprolactinemia,Inflammation,Oxidative Stress,Resveratrol}, url = {https://ejh.journals.ekb.eg/article_15894.html}, eprint = {https://ejh.journals.ekb.eg/article_15894_68b832cba3c42763ada1df0c95c2095e.pdf} } @article { author = {Mousa, Ayman and El-Mahalaway, Abeer and El-Desoky, Rania}, title = {The protective effects of sodium hyaluronate on corneal injuries induced by chlorinated water in adult male rabbits: A histological and immunohistochemical study}, journal = {Egyptian Journal of Histology}, volume = {41}, number = {2}, pages = {140-149}, year = {2018}, publisher = {Egyptian Society of Histology and Cytology in Cooperation with The Egyptian Knowledge Bank (EKB).}, issn = {1110-0559}, eissn = {2090-2417}, doi = {10.21608/EJH.2018.13835}, abstract = {Introduction: Chlorinated-water-induced corneal wounds lead to reduced transparency and disability of the cornea. Sodium hyaluronate (SH) is a natural lubricant with unique hygroscopic properties that control tissue hydration for topical eye care with excellent use as an ophthalmic product.Aim of the work: To evaluate the protective effects of sodium hyaluronate on corneal injuries induced by chlorinated water in adult male rabbits.Materials and Methods: A total of 20 adult male rabbits were divided equally into four groups treated with 2 drops of sodium hyaluronate on the corneal surface of both eyes two times daily for one month as follow: Group I (G1) was control group treated with distilled water, group II (G2) treated with chlorinated water, group III (G3) treated with SH while, group IV (G4) was treated with 2 drops of SH then 2 drops of chlorinated water. The corneas were excised and processed for histological and immunohistochemical study.Results: G2 showed epithelial thinning with some areas of cellular ulceration. Disturbance of normal stromal architecture, wide separation of collagen fibers and few degenerated keratocytes were noticed. In addition, a significant decrease of BCL-2 immunoexpression was observed in the cytoplasm of corneal epithelial cells (P<0.01). G3 and G4 showed improvement in the histological and immunohistochemical changes described in G2.Conclusion: Chlorinated water was harmful to the cornea and SH eye drops proved to minimize these corneal complications. Therefore, application of SH eye drops before swimming is recommended to flush chlorine drops away and to allow the tear film to remain over the cornea.}, keywords = {Chlorine,cornea,sodium hyaluronate}, url = {https://ejh.journals.ekb.eg/article_15895.html}, eprint = {https://ejh.journals.ekb.eg/article_15895_b61d6b873e31650affcc796ea7c24e93.pdf} } @article { author = {El-azab, Nahla and Mansy, Aisha and El-Mahalaway, Abeer and Sabry, Dina}, title = {Comparative study of the therapeutic effect of pomegranate alone or in combination with bone marrow mesenchymal stem cells on experimentally induced gastric ulcer in adult male rats: A histological and immunohistochemical study}, journal = {Egyptian Journal of Histology}, volume = {41}, number = {2}, pages = {150-166}, year = {2018}, publisher = {Egyptian Society of Histology and Cytology in Cooperation with The Egyptian Knowledge Bank (EKB).}, issn = {1110-0559}, eissn = {2090-2417}, doi = {10.21608/EJH.2018.13838}, abstract = {therapeutic effects. Bone marrow mesenchymal stem cells (BMSCs) are a novel approach with promising therapeutic potential for curing human diseases.Aim of the work: To evaluate the effect of pomegranate and BMSCs on experimentally induced gastric ulcer in adult male rats.Materials and Methods: Sixty adult male rats were divided into four groups. Group I kept as the control group. Group II (Gastric ulcer), rats received 100% ethanol orally by gastric tube. Group (III) subdivided into 3 subgroups. Subgroup IIIa included rats with gastric ulcer that treated with pomegranate only. Subgroup IIIb, rats with gastric ulcer treated with BMSCs alone. Subgroup IIIc; gastric ulcer rats treated with a combination of pomegranate and BMSCs. Group IV included the untreated gastric ulcer rats. Fundus of stomach specimens were prepared and examined using histological and immunohistochemical techniques.Results: Groups II and IV showed various changes as deep erosion, marked damage of most of the cells. The presence of the inflammatory cells in gastric mucosa were supported by a significant increase of tumor necrosis factor α (TNF-α) expression and collagen fibers depositions (P<0.01). A significant decrease of Periodic Acid Schiff (PAS) positive reaction and vascular endothelial growth factor (VEGF) expression was observed (P<0.01). Subgroup IIIa showed attenuation of some histological changes, while subgroups IIIb and IIIc revealed more improvement of the histological and immunohistochemical changes described before.Conclusion: BMSCs can ameliorate experimentally-induced gastric injury in rats and protect the gastric tissue. Addition of pomegranate to BMSCs can lead to better results.}, keywords = {Bone marrow mesenchymal stem cells (BMSCs),Gastric ulcer,pomegranate,tumor necrosis factor-α,vascular endothelial growth factor (VEGF)}, url = {https://ejh.journals.ekb.eg/article_15897.html}, eprint = {https://ejh.journals.ekb.eg/article_15897_53840396f9f3b38fe1928e3adf8d8cb1.pdf} } @article { author = {Elwan, Walaa and Ragab, Ayah and Ragab, Mohamed}, title = {Histological and immunohistochemical evaluation of the dose-dependent effect of gold nanoparticles on the renal cortex of adult female albino rat}, journal = {Egyptian Journal of Histology}, volume = {41}, number = {2}, pages = {167-181}, year = {2018}, publisher = {Egyptian Society of Histology and Cytology in Cooperation with The Egyptian Knowledge Bank (EKB).}, issn = {1110-0559}, eissn = {2090-2417}, doi = {10.21608/EJH.2018.13839}, abstract = {Introduction: Applications of gold nanoparticles (GNPs) in modern life lead to the release of GNPs into the environment, thus representing a potential hazard to human health.Aim of the work: To evaluate the effect of GNPs on the renal cortex of adult female rats employing different histological and immunohistochemical techniques.Materials and Methods: Thirty adult female albino rats were divided into three equal groups; Group I (Control), in groups II and group III, GNPs were administered daily as 40μg/kg and 400μg/kg for 14 days, respectively. Specimens were processed for light and electron microscopy, in addition to immunohistochemical staining for tumor necrosis factor-alpha (TNF-α).Results: GNPs-treated animals showed many hypercellular glomeruli. Most proximal convoluted tubules (PCTs) were severely distorted, their cells showed vacuolated cytoplasm and pyknotic nuclei. Many distal convoluted tubules (DCTs) showed exfoliated cells and some karyolytic nuclei. Some tubules were totally distorted. Dilated congested blood vessels together with extensive mononuclear cellular aggregations were observed. Ultrastructural examination revealed podocytes with small heterochromatic nuclei and interrupted processes with distortion and effacement. Many GNPs resided in the podocytic terminal processes. Some cells of PCTs showed irregular nuclei, variably sized and shaped mitochondria with loss of their basal orientation. Focal areas of rarefied cytoplasm and apical vacuoles were observed. Cells of DCTs showed irregular nuclei and numerous abnormally shaped mitochondria. Irregularly thickened basal lamina and GNPs were clearly observed in their cytoplasm. The immunohistochemical study showed a highly significant increase in TNF-α immunoreaction.Conclusion: GNPs cause dose-dependent structural changes in renal cortex with various degrees of inflammation.}, keywords = {electron microscopy,Gold nanoparticles,renal cortex,TNF-α}, url = {https://ejh.journals.ekb.eg/article_15899.html}, eprint = {https://ejh.journals.ekb.eg/article_15899_3f76e09ed80943841eb00e7dec9e3149.pdf} } @article { author = {Kassab, Amira}, title = {Wheat germ oil attenuates deltamethrin-induced injury in rat cerebellar cortex: Histological and immunohistochemical study}, journal = {Egyptian Journal of Histology}, volume = {41}, number = {2}, pages = {182-191}, year = {2018}, publisher = {Egyptian Society of Histology and Cytology in Cooperation with The Egyptian Knowledge Bank (EKB).}, issn = {1110-0559}, eissn = {2090-2417}, doi = {10.21608/EJH.2018.13840}, abstract = {Introduction: Deltamethrin, a widely used insecticide, has neurotoxic effects causing apoptosis. Wheat germ oil is a highly valuable part of wheat that proved to have neuro-protective and anti-apoptotic effects.Aim of the work: To evaluate the possible protective role of wheat germ oil in deltamethrin-induced injury in rat cerebellar cortex.Materials and Methods: Four equal groups of adult male albino rats "10 rats, each" were used; control group, wheat germ oil (WGO) group (100 mg/kg), deltamethrin group (0.6 mg/kg), and deltamethrin & wheat germ oil combination group. Daily oral doses were given for consecutive thirty days. Specimens of the cerebellum were processed for histological and immunohistochemical study. Morphometric studies and statistical analysis were performed.Results: In comparison with the control group, the specimens of the deltamethrin group showed disturbed monolayer arrangement of Purkinje cells with loss of the normal shape. Shrunken deeply stained Purkinje cells as well as some lost cells were noticed. Immunohistochemically, a significant decrease in Bcl2 immunoreaction revealed together with a significant increase in Bax immunoreactivity in Purkinje cells. Moreover, significant increase in the GFAP immune-expression was seen in all cortical layers. In contrast, minimal changes appeared in the group treated concomitantly with deltamethrin and wheat germ oil.Conclusion: Wheat germ oil attenuates deltamethrin-induced structural changes in rat cerebellar cortex.}, keywords = {cerebellar cortex,Deltamethrin,Wheat germ oil}, url = {https://ejh.journals.ekb.eg/article_15900.html}, eprint = {https://ejh.journals.ekb.eg/article_15900_6228f1f952b4970dda4a7868e511871f.pdf} } @article { author = {Ismail, Dalia and Aboulkhair, Alshaymaa}, title = {Royal jelly protects against experimentally-induced ulcerative colitis in adult male albino rats : A histological study}, journal = {Egyptian Journal of Histology}, volume = {41}, number = {2}, pages = {192-203}, year = {2018}, publisher = {Egyptian Society of Histology and Cytology in Cooperation with The Egyptian Knowledge Bank (EKB).}, issn = {1110-0559}, eissn = {2090-2417}, doi = {10.21608/EJH.2018.13841}, abstract = {Introduction: Ulcerative colitis (UC) is a chronic inflammatory condition characterized by acute episodes of colonic inflammation. Its pathogenesis is associated with decreased antioxidant capability. Royal Jelly (RJ) is usually used as a complementary therapy in various diseases because of its antioxidant, anti-inflammatory and immunomodulatory effects.Aim of the work: To evaluate the protective effect of RJ against acetic acid-induced UC in adult male albino rats.Materials and Methods: Thirty rats were divided equally into 3 groups. Group I was the control group. Group II included rats subjected to intracolonic acetic acid (AA) for induction of UC. Group III 10 rats treated with RJ (250 mg/kg/day orally) for 7 days, thereafter subjected to AA. RJ was administered for another 14 days. Rats were sacrificed after 21 days. Serum glutathione (GSH) and malondialdehyde (MDA) were assessed. Colonic sections were subjected to H&E, Periodic Acid Schiff (PAS), toluidine blue, Mallory's trichrome stains and cyclooxygenase-2 (COX-2) immunohistochemical stain.Results: Group II showed significant decrease in GSH, significant increase in MDA and marked histological alterations in colon. There was a significant decrease in the number of goblet cells, but significant increases in the number of mast cells, area% of collagen fibers and COX-2 immunoexpression compared to the control. In group III, GSH was significantly increased and MDA was significantly decreased compared to group II. The colon showed minimal changes, significant increase in the number of goblet cells, significant reduction in the number of mast cells, collagen deposition and COX-2 immunoreactivity compared to group II. When compared to the control, there was no significant difference regards the serological, histological and morphometric results, except for the number of goblet cells that revealed significant decrease.Conclusion: RJ proven to protect against acetic acid-induced UC in albino rats.}, keywords = {acetic acid,Inflammation,Oxidative Stress,royal jelly,ulcerative colitis}, url = {https://ejh.journals.ekb.eg/article_15902.html}, eprint = {https://ejh.journals.ekb.eg/article_15902_be4cbaa903146f299275a64e968edfcd.pdf} } @article { author = {Omar, Abeer and Aboulkhair, Alshaymaa}, title = {Do microvesicles derived from adipose mesenchymal stem cells have a therapeutic potential on Escherichia coli lipopolysaccharides-induced sepsis in Zona fasciculata of adult male albino rats? A histological study}, journal = {Egyptian Journal of Histology}, volume = {41}, number = {2}, pages = {204-219}, year = {2018}, publisher = {Egyptian Society of Histology and Cytology in Cooperation with The Egyptian Knowledge Bank (EKB).}, issn = {1110-0559}, eissn = {2090-2417}, doi = {10.21608/EJH.2018.13843}, abstract = {Introduction: Sepsis is a major health problem with high mortality rate, despite advanced medications. Although hypothalamic-pituitary-adrenal (HPA) axis activation can control its destructive inflammatory reactions, adrenal insufficiency (AI) is commonly associated with it. Mesenchymal Stem Cell-Derived Microvesicles (MSCs-MVs) were proved to be as effective as their origin cells in tissue repair.Aim of the work: To study the effects of Escherichia coli-lipopolysaccharides (E. coli-LPS) induced sepsis on Zona fasciculata of adult male albino rats and probable therapeutic effects of adipose derived mesenchymal stem cells-microvesicles (ADMSCs-MVs).Materials and Methods: Forty-four adult male albino rats were divided into three groups; (I) the controls, (II) LPS and (III) MV. Sepsis was induced in LPS and MV-groups using E. coli-LPS. Four hours later, MV-group received single intravenous (IV) injection of PKH26 labelled ADMSCs-MVs. All animals were sacrificed 48 hours after ADMSCs-MVs administration. Tumor necrosis factor-α (TNF-α), cyclo-oxygenase-2 (COX-2) and nitric oxide (NO) levels were measured in adrenal homogenates of each group. Sections from all groups were subjected to H&E, besides, immunohistochemical stains for CD44, inducible nitric oxide synthase (iNOS) and proliferating cell nuclear antigen (PCNA). Unstained sections of subgroup IC and MV-group were examined by fluorescent microscope. Mean number of CD44 and PCNA positive cells and mean area percent of iNOS immunoreaction were measured and data were statistically analyzed.Results: LPS-group showed non-survivors (24 hours after LPS-injection) and survivors with features of Zona fasciculata inflammatory damage, besides increased TNF-α and NO and decreased COX-2. The MV-group revealed marked increase in CD44 and PCNA, but significant decrease in iNOS immunoreactions. Additionally, ADMSCs-MV homing was detected in Zona fasciculata.Conclusion: LPS-induced sepsis caused marked Zona fasciculata inflammatory degeneration improved by ADMSCs-MVs single IV injection; mostly through transfer of proteins, mRNA, microRNA and/or organelles with reparative functions from ADMSCs to the injured tissues.}, keywords = {ADMSCs-MV,CD44,COX-2,iNOS,Sepsis,Zona fasciculata}, url = {https://ejh.journals.ekb.eg/article_15904.html}, eprint = {https://ejh.journals.ekb.eg/article_15904_610763217f925509968c79617246f1c2.pdf} } @article { author = {Aboul-Fotouh, Gihan and Abou El-Nour, Rahma and Farag, Eman and Boughdady, Wafaa}, title = {Histological study on the possible protective effect of curcumin on potassium dichromate induced hypothyroidism in adult male albino rats}, journal = {Egyptian Journal of Histology}, volume = {41}, number = {2}, pages = {220-235}, year = {2018}, publisher = {Egyptian Society of Histology and Cytology in Cooperation with The Egyptian Knowledge Bank (EKB).}, issn = {1110-0559}, eissn = {2090-2417}, doi = {10.21608/EJH.2018.13844}, abstract = {Introduction: Potassium dichromate, a widely used heavy metal in several industries induces hypofunction and tissue insult of the thyroid gland via oxidative stress. Curcumin; is a natural commonly used spice has a strong anti-inflammatory and antioxidant properties.Aim of the work: To investigate the possible protective effect of curcumin on the hypothyroidism induced by potassium dichromate in albino rats.Materials and Methods: Thirty- five adult male albino rats were divided into five groups, 15 rats in group I (control) and 5 rats in each of group II, III, IV and V. Group II (curcumin group) received curcumin orally (100 mg /kg/bw) daily for 4 weeks. Group III (potassium dichromate induced hypothyroidism) received i.p injection of potassium dichromate (2 mg/kg/bw) daily for 2 weeks. Group IV (recovery group) received potassium dichromate as group III then left untreated for another 2 weeks. Group V(curcumin and potassium dichromate group) received curcumin concomitant with potassium dichromate as in groups II and III, respectively daily for 2 weeks and only curcumin was continued for another 2 weeks. T3, T4 and TSH were assessed. Thyroid sections were subjected to toluidine blue, H&E, PAS and PCNA immunohistochemical stains. Morphometric and statistical studies were done.Results: Thyroid tissue insult and hypofunction with significantly decreased T3, T4 and increased TSH were detected in group III. Additionally, there was a significant increase in the mean values of follicular cell height, follicular diameter, mean number of PCNA positive nuclei and a significant decrease in the mean value of area percent of colloid versus group I, II and V with non-significant differences versus group IV. In group V, there was an obvious serological and histological improvement compared to group III and IV.Conclusion: Curcumin had protective effect against hypothyroidism and thyroid tissue damage induced by potassium dichromate.}, keywords = {Curcumin,hypothyroidism,PCNA,Potassium Dichromate,rat,THYROID}, url = {https://ejh.journals.ekb.eg/article_15905.html}, eprint = {https://ejh.journals.ekb.eg/article_15905_fab66b4bcff44ad30858e9dcde6e1794.pdf} }